The primary purpose of the Research Assistant II position is to apply technical expertise and experience to coordinate, design, and carry out cancer research projects while following standard operating procedures for the laboratory.

JOB SPECIFIC COMPETENCIES

Immunologic Assays and Techniques Assist in standard immunology techniques (ELISA, ELISpot, flow cytometry, target cell killing assays), T-cell culture and expansion, dendritic cell generation and function. Perform magnetic assisted cell isolation, flow assisted cell sorting. Assist in data analysis and interpretation (FlowJo, Prism, etc).

Molecular Biology Assays and Techniques Orders and organizes supplies for the laboratory. Perform nucleic acid extraction (DNA, RNA, etc.), PCR analyses, immunoblotting, next generation sequency library preparations. Familiarity with routinely used instruments (Bioanalyzer, Qubit, etc). Assist in single cell sample processing and preparation.

Cell Culture and Good Lab Practices Maintains lab stock cancer cell lines for cell culture and mouse xenograft studies, to include: growing, expanding, and freezing utilizing sterile techniques. Ability to carry out and learn new in vitro and in vivo assays in the field of molecular biology, biochemistry, and cell biology. Some procedures will require the use of hazardous substrates. Must maintain appropriate environmental surveys for radioactivity and other hazardous materials. Must maintain records of hazardous chemicals and reagents as required. Handles patient tissues, with familiarity of safety procedures. Other duties as assigned.

EDUCATION:

Required: Bachelor's degree with major course works in one of the basic sciences.

Preferred: Master's degree with major course work in one of the basic sciences or Medical degree.

EXPERIENCE:

Required: One year experience in scientific or experimental research work. No experience required with preferred degree.

Preferred: None.

It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html