Senior Associate Scientist, High-Throughput Protein Production
Pfizer's BioMedicine Design (BMD) High-Throughput Protein Production (HTP) group is seeking a highly motivated scientist to manage and perform antibody expression and purification, from hundreds to thousands of proteins per week. The candidate will play a critical role in production and automation process innovation in a core group that is responsible for high-throughput cloning, expression & purification of antibodies (microgram-to-milligram scale).
The candidate will be part of a team responsible for high throughput automated transfection, purification, quantitation, analytical assessment, and normalization/delivery of protein samples, including conventional antibodies, bispecifics, and occasional non-antibody proteins; data upload in the departmental database/ELNs; and interacting with project teams. The candidate will contribute to group-wide deliverables, work on several projects simultaneously, explore new purification technologies, and present results and updates at group, project, and departmental meetings.How You Will Achieve It
- High-throughput automated antibody purification, quantitation, analytical SEC, normalization, and redox for bispecific antibody generation utilizing the team's liquid handling automation equipment.
- Optimization of automated processes throughout the HTP workflow
- Maintaining mammalian cell stocks, transfecting and harvesting conditioned medium in small- and micro-scale.
- Managing the purification database, scheduling purifications, and delivering custom arrayed materials to downstream users
- Maintaining & troubleshooting of liquid handlers
- Development of automated bispecific antibody generation methods using different antibody formats
- Close interaction with protein engineering project teams to plan and coordinate protein production
- Presentation of purification data at group & project team meetings
- Regular review of literature to m aintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods
- Bachelor's degree & 3+ years of experience, or master's degree with 0+ years of experience, in protein purification, biochemistry, biology, chemical engineering or a related discipline, along with a basic understanding of antibodies and protein production.
- Demonstrated technical proficiency in protein purification.
- At least 2 years of experience with using laboratory automation, including writing and troubleshooting protocols.
- Track record of strong problem solving and organizational skills, orientation to detail
- A bility to multi-task, prioritize responsibilities, work under strict deadlines, and remain flexible with changing needs.
- Ability to work effectively to solve process development problems and implement improved procedures.
- Strong written and oral communication skills
- Experience with Tecan, Beckman, and/or Lynx liquid handling platforms
- Industry experience in protein expression/purification/antibodies/ molecular biology
- Experience with high throughput sample tracking and laboratory logistics software
- Experience in writing and understanding methods for laboratory automation technologies.
Other Job Details:
- Lifting, sitting, standing, walking, bending, reaching, ability to perform mathematical calculations and ability to perform complex data analysis.
- Last Day of application: February 9th, 2023
- Work Location Assignment:On Premise, colleagues work in a Pfizer site because it's needed to get their job done. They may have flexibility to work remotely from time to time, but they are primarily on-site.
Relocation assistance may be available based on business needs and/or eligibility.
\nPfizer requires all U.S. new hires to be fully vaccinated for COVID-19 prior to the first date of employment. As required by applicable law, Pfizer will consider requests for Reasonable Accommodations.Sunshine Act
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Research and Development