Postdoctoral Fellow

CRISPR Genome Surgery in preclinical humanized mice and patient-specific stem cells
The Barbara & Jonas Stem Cell and Regenerative Medicine Laboratory in the Departments of Ophthalmology, Pathology & Cell Biology at Columbia University seeks postdoctoral applicants for research with patient-specific stem cell lines: disease modeling, repair, transplantation as well as gene therapy. Other than cancer, blindness is the most feared condition among Americans. Our NIH-R01EY018213, R24EY026682, R01EY024698 and R01EY026682 supported laboratory investigates cell-based treatments for neurodegeneration and develops patient-specific disease models relevant to Investigational New Drug (IND) Application – FDA. The eye is an ideal testing ground for stem cell therapies because of its relative immune privilege and its ready accessibility for monitoring and imaging purposes.

Books: Precision Medicine, CRISPR, and Genome Engineering: Moving from Association to Biology and Therapeutics. Stephen H. Tsang (Ed). Adv Exp Med Biol. 2017;1016:1-178. Springer Nature Publishing Group;
Stem Cell Biology and Regenerative Medicine in Ophthalmology, Springer Press, NY, 2012;  

References on Genome Surgery:
Molecular Therapy, 2022;30(4) Pages 1407-1420. PMID: 35150888.
The Journal of Clinical Investigation 2018;128(6):2177-2188.
Ophthalmology 2018;125(9):1421-1430.
Mol Ther. 2016;24(8):1388-1394.

References on Stem Cells:  
Human Molecular Genetics. 2014 Jan 31. PMID: 24497574;
Transplantation. 89(8), 911-9. Mol Med. 2012 18:1312-9.

References on Gene Therapy:
Proc Natl Acad Sci U S A. 2017;114(20):5259-5264.
J Clin Invest. 2018;128(6):2177–2188. J Clin Invest. 2015 Sep 1;125(9):3704-13.
Human Molecular Genetics. PMID: 24101599; PMID: 23108158;
J Neurosci. PMID: 23946405.
Science. 272, 1026-1029  PMID: 8638127; Science. 282, 117-21. PMID: 9756475

References on metabolome reprogramming:
J Clin Invest. 2016;126(12):4659-4673 PMID:27841758. Hum Mol Genet. 2016 Aug 11. pii: ddw256. PMID: 27516389

Requirements: 

Applicants should have a PhD in enzyme kinetics, electrophysiology, cell biology, molecular biology or related fields. Please email your CV to crispr.ips@gmail.com.

Duties:
Work within the guidelines to implement genetic research projects.
Drafting of research abstracts, manuscripts, posters, manuscripts, and preparation and presentation of research findings.

Successful candidates should have:
Broad knowledge and excellent skills in a wide-variety of molecular biology methods from very fundamental to advanced technologies, including tissue culture (cell line, primary cell, stem cell, mouse handling, mouse model creating (transgene, knock-in, knock-out), gene editing techniques (CRISPR, TALEN, Zinc finger) in vitro and in vivo, etc. 

Willingness to learn rapidly evolving scientific knowledge and new techniques;
Ability to work with peers as well as outside of the team to enable higher performance;
Ability to effectively convey information verbally and in writing;