Research Assistant 

Employer
Case University
Location
Cleveland, Ohio (US)
Salary
Commensurate with experience
Closing date
Nov 29, 2022

View more

Discipline
Life Sciences, Biochemistry
Position Type
Full Time
Job Type
Research Scientist
Organization Type
Academia
You need to sign in or create an account to save a job.

We have an immediate need for a seasoned Research Assistant with at least 5 years experience as a Technician

POSITION OBJECTIVE

Working with a high degree of independence and under general direction, the Research Assistant 4 will coordinate major activities in protein biochemistry/biophysics and related technical areas in the laboratory. Key responsibilities include daily protein production for the various projects of the laboratory, record keeping, experimentation, and support of lab and postdoctoral personnel with orders, reagents, and buffers etc.

ESSENTIAL FUNCTIONS

  1. Coordinate major activities of laboratory/ research. Solely responsible for protein production and enzymatic and biophysical assays for activity in the laboratory. Harvest overnight bacterial/yeast/insect cell cultures expressing recombinant protein, and process for protein purification using lab protocols. Place purified proteins in dialysis for further purification steps the following day. Conduct enzymatic and biochemical/biophysical assays with purified proteins and lipids (liposomes, lipid nanotubes etc.). Responsible for lab organization, vendor research, ordering, and maintaining laboratory (e.g., freezer) stocks. (30%)
  2. Perform the most complex quantitative analytical procedures utilizing scientific software (e.g., Origin, Graphpad Prism) for data analyses regarding protein functionality, folding, protein-lipid and protein-protein interactions. Perform high level calculation of data, interpret data and make recommendations to the principal investigator regarding protocol improvement and reproducibility. (30%)
  3. May supervise research assistants, students, and technicians as well as produce proteins and perform assays for other lab personnel. (10%)
  4. Assist in developing and improving protocols for current and new proteins that the lab chooses to work on. Coordinate information with others as needed. (10%)
  5. Teach or instruct others in research techniques including protein expression, purification and characterization as necessary. (10%)
  6. Co-author research projects. Write detailed protocols, methods, and produce figures for publication. (6%)

 REQUIREMENTS

Experience: 5 to 8 years of related experience required.

Education: Bachelor’s degree in Biochemistry or related fields required. Master’s degree preferred.

REQUIRED SKILLS

  1. Experience as a skilled biochemist with vast experience in protein production (recombinantly from E. coli, yeast, insect cells etc.), protein purification, and various chromatography techniques.
  2. Experience in the use of chromatography instrumentation and other standard, common laboratory equipment.
  3. Experience in the use of AKTA and Biorad chromatography systems.
  4. Basic protein characterization skills such as SDS-PAGE, absorbance and CD spectroscopy is an absolute must.
  5. Has knowledge of commonly used concepts, practices, and procedures within a particular field.
  6. Relies on instructions and pre-established guidelines to perform the functions of the job.
  7. Ability to operate laboratory equipment.
  8. Ability to meet consistent attendance.
  9. Ability to interact with colleagues, supervisors and customers face to face.

WORKING CONDITIONS

General laboratory environment. May have exposure to lab chemicals. Personal protective equipment, including gloves, lab coats, and goggles will be provided to limit exposure to hazards.

Please forward your resume  to rxr275@case.edu

 

Apply for Research Assistant 

Fields marked with an asterisk (*) are required

Your file must be a .doc, .pdf, .docx, or .rtf. No larger than 1MB
Selected file:

Supplementary Documents

Your file must be a .doc, .pdf, .docx, or .rtf. No larger than 1MB
Selected file:
Your communication preferences

When you apply for a job we will send your application to the named employer, who may contact you. By applying for a job listed on Science Careers you agree to our terms and conditions and privacy policy. You should never be required to provide bank account details. If you are, please contact us. All emails will contain a link in the footer to enable you to unsubscribe at any time.

You need to sign in or create an account to save a job.

Get job alerts

Create a job alert and receive personalized job recommendations straight to your inbox.

Create alert