Staff Scientist - Cell Culture

City of Hope
Duarte, California
Closing date
Dec 16, 2022

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Job Details

About City of Hope

City of Hope is an independent biomedical research and treatment organization for cancer, diabetes, and other life-threatening diseases.

Founded in 1913, City of Hope is a leader in bone marrow transplantation and immunotherapy such as CAR T cell therapy. City of Hope's translational research and personalized treatment protocols advance care throughout the world. Human synthetic insulin, monoclonal antibodies and numerous breakthrough cancer drugs are based on technology developed at the institution. AccessHope™, a subsidiary launched in 2019 serves employers and their health care partners by providing access to City of Hope's specialized cancer expertise.

A National Cancer Institute-designated comprehensive cancer center and a founding member of the National Comprehensive Cancer Network, City of Hope is ranked among the nation's "Best Hospitals" in cancer by U.S. News & World Report and received Magnet Recognition from the American Nurses Credentialing Center. Its main campus is located near Los Angeles, with additional locations throughout Southern California and in Arizona.

City of Hope's commitment to Diversity, Equity, and Inclusion

We believe diversity, equity and inclusion is key in serving our mission to provide compassionate patient care, drive innovative discovery, and advance vital education focused on eliminating cancer and diabetes in all our communities. Our commitment to Diversity, Equity and Inclusion ensures we bring the full range of skills, perspectives, cultural backgrounds, and experiences to our work -- and that our teams align with the people we serve to build trust and understanding. We are dedicated to fostering a community that embraces diversity - in ideas, backgrounds, and perspectives; this is reflected in our work and represented in our people.

Position Summary

We are looking for a talented Staff Scientist with relevant cell culture, protein purification, and protein-based assay experience. Specific technical experience includes: ELISA, Immunoprecipitation, immunohistochemistry, Western blotting, 1D- and 2D-polyacrylamide gel electrophoresis, affinity chromatography, FFPE tissue preparation for IHC, Optimizing antibody staining of FFPE tissue sections, familiarity with hematological staining methods, microscopy, photo-documentation and quantification of immuno-stained tissues.

Specific experience in the growth and maintenance of hybridoma cell lines, subcloning of cells in culture, including transfected human cultured cells, sterile technique, purification of antibody secreted by the cell into the media, growth of cells in serum-free media, walking cells into serum free media, and specific/selective immuno-staining of prepared sections FFPE tissue sections is preferred.

Basic education, experience and skills required for consideration:
  • PhD in life sciences or related field. Experience may substitute for the education required.
  • Familiarity with skills and experience above is required.

Additional Information:

As a condition of employment, City of Hope requires staff to comply with all state and federal vaccination mandates.

For more information on Dr. Robert Hickey, please visit here .

City of Hope is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, or status as a qualified individual with disability.


City of Hope, an innovative biomedical research, treatment and educational institution with over 5,000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy.

Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research.  City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation’s 20 leading cancer centers that develops and institutes standards of care for cancer treatment.

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