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HTPD Downstream Associate

Spark Therapeutics
Philadelphia, Pennsylvania. Hybrid and remote options available for select opportunities.
Salary not provided
Closing date
Jun 28, 2022

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Life Sciences, Biotechnology
Organization Type
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Job Details

The downstream and formulation process development team is seeking a highly motivated scientist to work in high-throughput process development (HTPD) for gene therapy. This is a unique opportunity to work with advanced HTPD and automation tools, and cutting edge Adeno-Associated Viral Vectors. The position resides within the HTPD core of the Downstream and Formulation Process Development team.

The associate will work with experienced scientists to use automation tools for high-throughput chromatography and downstream experimentation. Key focus areas will include (a) chromatography optimization, (b) chromatography robustness, and (c) HT material purification to support partner groups such as cell culture and cell line development. Strong collaboration skills will be important when working with multiple stakeholders and multiple projects. General preparative and analytical chromatography experience with biologics is required. Strong quantitative skills (DOE and similar) are required to work with and interpret multifactor datasets. Familiarity with lab automation (Tecan/Hamilton), programming (Python/Matlab), and gene therapy is preferred but not required.

Key responsibilities include:

  • Work with HTPD core team members to execute key chromatography studies
  • Collaboration with upstream and cell line development colleagues to purify material to support bioreactor optimization.
  • Statistical analysis (JMP, DOE) to analyze key datasets
  • Keep accurate experiments records and write reports to summarize data


% of Time

Job Function and Description


Chromatography optimization: Use HTPD techniques to optimize and assess robustness of key chromatography manufacturing steps


Support upstream and cell-line development work by using HTPD chromatography techniques to purify materials for quality determination


Analytical testing (HPLC, DLS, and similar) of material from key experiments


Collaboration with automation and data scientists to improve automation workflows


Effective communication of work through reports and presentations.

Education and Experience Requirements


  • BS (+2years relevant experience), in chemical engineering, bioengineering, biochemistry, or related area.
  • Experience with preparative and analytical chromatography (Akta, HPLC)
  • Experience with purification of biologics (proteins, vaccines, viral vectors)
  • Familiarity with multifactor statistics (DOE, JMP, or similar)

  • Experience with chromatography automation (Robocolumns, Tecan, Protein Maker)
  • Experience with downstream process development (chromatography, filtration) of viral vectors (AAV preferred).

Key Skills, Abilities, and Competencies

  • Hardworking, proactive, creative thinker
  • Able to juggle multiple parallel objectives
  • Strong quantitative skills
  • Ability to collaborate with coworkers across a wide range of backgrounds

Complexity and Problem Solving

  • Design and execute HTPD studies
  • Work with collaborators to reliably handle samples and communicate data
  • Work with HTPD team to improve and standardize workflows for efficient execution and analysis of experiments

Internal and External Contacts

  • Fellow HTPD team members: to execute key studies and improve HTPD workflows
  • Downstream process development and downstream manufacturing science: to execute key studies for process development
  • Automation team: to implement and improve key new workflows
  • Cell line development and upstream colleagues: to purify and determine quality of key samples

Other Job Requirements

Are there any other requirements such as below:

  • Must be able to lift 25 pounds
  • Must be able to wear appropriate clean room attire and all Personal Protective Equipment

Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.

Nearest Major Market: Philadelphia

Join the Spark Team

We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable - until now.

We don't follow footsteps. We create the path.


At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.

Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.

Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.

Find Us
3737 Market Street
Suite 1300
United States
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