- Specific experimental tasks : Conducting routine experiments including ELISA assays, ELISPOT assays, western blot analysis, immunoprecipitation, gene knockdown, transfection/transduction of mammalian /bacterial cells, proliferation/apoptosis study, gene expression library construction, RT-PCR, immunostaining of tissue sections, gel electrophoresis of proteins and nucleic acids, mammalian cell culture, T-cell isolation and culture, cryopreservation of cells, preparation of DNA, RNA, and protein from cells, blood, tissues, and FFPE slides for next-generation sequencing , microarray, methylation array, and protein array analyses. Data analyses from DNA/RNA/protein arrays and next-generation sequencing. Animal experiments including testing the efficacy and mechanisms of action of specific anti-cancer agents both in vivo and ex vivo according to IRB-approved animal protocols.
- Media and reagent preparation: Measure optical density by spectrophotometer and calculate sample concentrations. Prepare solution and buffer based upon different characteristics of solvents and chemicals. Must exercise proper caution when handling toxic, hazardous, flammable or potentially carcinogenic agents.
- Ordering supplies and maintenance of equipment: Periodically reviews and place orders for laboratory supplies including community reagents and chemicals. Assist in assembly, operation, and maintenance of laboratory.
- Tissue culture and handling of hazardous biological materials: Maintains and establishes tissue culture cell lines with good sterile techniques; determine cell counts on the hemocytometer or coulter counter, perform colony counts and determine cell viability. Prepares sterile medias and solutions. Prepares cells for cryogenic storage and re-establishment by the use of small vials, dry ice and liquid nitrogen. Precaution against blood-borne pathogens must be taken whenever human-derived cells are used in accordance with the institutional Universal Precautions Plan. Must demonstrate and practice laboratory safety precautions. Must manually handle, store and dispose of radioactive isotopes, chemically hazardous materials, and biohazardous materials in procedures specified to meet MDACC safety guidelines. Accurately records use and disposal of all radioactive materials. The position requires the maintenance and analysis of various viable (human) cell lines established from human tumors, immortalized human cell lines (endothelial) and primary human cell lines (umbilical vein endothelial cells). Tests may periodically be performed for mycoplasma contamination. Requires use of single and multichannel pipettors. Fine finger dexterity will be required to manipulate small tissue culture vials.
- Guide, teach, supervise, and assist students, collaborators, and laboratory members to accomplish research.
- Write manuscripts, research proposals and apply for grant funding, and collaborate with laboratory members to reinforce expertise and advance research objectives including the publication of articles and presentations at scientific meetings
- Other duties as assigned.
- The above functions require a strong command of the English language, including spelling, writing, and the ability to clearly and accurately communicate verbally in English both face-to-face and over the phone.
Education Required: Bachelor's degree in one of the natural sciences or related field. Experience Required: Three years of experience in scientific or experimental research work. With preferred degree, one year of required experience.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html