Research Technician III - Translational Leukemia Research

Summary

Dr. Rachel Rau of Baylor College of Medicine/Texas Children's Hospital is seeking a research technician to work in her translational hematologic malignancies lab. Dr. Rau's lab is focused on understanding the genetic and epigenetic mechanisms through which leukemias develop and develop resistance to currently used therapies. The ultimate goal is to uncover novel therapeutic avenues for difficult to treat leukemias. For this work, the lab employs genetically engineered murine modeling, human leukemia cell lines and primary samples, genome engineering with CRISPR/Cas9 technology and next generate high throughput sequencing modalities. Current projects in the include work to understand how mutations of epigenetic regulators such as DNMT3A, cohesin, and TET2 collaborate with co-occurring signaling pathway and transcription factor lesions to drive leukemia. We are also interested in understanding how individuals with germline mutations of DNMT3A contribute to blood perturbations including leukemia. Lastly, we are working to understand why certain subsets of leukemia are more refractory to standard therapy, and use that enhanced understanding to develop targeted chemosensitizing interventions.

The successful applicant for this position will be a motivated, organized, and team-oriented individual who is comfortable working with mice to model hematologic malignancies. Prior experience in flow cytometry, next generation sequencing library preparation, qPCR, immunoblotting/immunochemistry, CRISPR/Cas9, cell cycle analysis, and sterile tissue culture are preferred but not required.
Excellent knowledge of English, both oral and written, is required.

Job Duties

• Prepares and conducts laboratory research experiments.
• Conducts flow cytometry and compensation (with training).
• Extraction of RNA/DNA; next generation sequencing library preparation eg RNAseq, ChIPseq (with training).
• Sterile tissue culture.
• Mouse work (with training) including breeding and husbandry, harvesting bone marrow and other hematopoietic organs, bleeding and injecting.
• Thawing of irreplaceable primary patient samples (after training).
• Produces, analyzes and interprets reliable, consistent data.
• Conducts literature searches related to experiment conduct and presents and records data in order to support research related decision making.
• Accurately reports and documents all results of experiments.
• Constant evaluation of the success of experiments to improve lab related processes.
• Orders supplies as needed.

Minimum Qualifications

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• One year of relevant experience.

Preferred Qualifications

• Master's degree in a related field.
• Prior experience in flow cytometry, apoptosis assays, qPCR, immunoblotting/immunochemistry, cell cycle analysis,and murine models of disease are helpful but not required.
• Excellent knowledge of English, both oral and written.
• The successful applicant for this position will be a motivated, organized, and team-oriented individual.

Other

Research Technicians in my lab have the opportunity to serve as first author on abstracts and posters on project to which they are assigned primary researcher role. They are also granted co-authorship on other projects to which they contribute.

Baylor College of Medicine requires employees to be fully vaccinated -subject to approved exemptions-against vaccine-preventable diseases including, but not limited to, COVID-19 and influenza.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

11542

CA; SN