Research Associate_ RVC Assay

Spark Therapeutics
Philadelphia, Pennsylvania. Hybrid and remote options available for select opportunities.
Salary not provided
Closing date
May 31, 2022

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Health Sciences, Drug Development
Job Type
Research Scientist
Organization Type

Job Details

Primary Duties:

The successful candidate will execute established protocols for Research grade AAV vector characterization and release assays. the candidate will also assist therapeutic area scientists in cloning and synthesis of new DNA constructs for pre-clinical grade rAAV vector production.

The individual will participate in (but not be limited to):

  • rAAV vector characterization and QC release assays
  • execute plasmid preparation and release to support RVC vector production and translational research needs
  • Interact with therapeutic area scientists to coordinate plasmid preparation and QC
  • Assist in the development and optimization of analytical assays
  • Maintain relevant laboratory supplies and equipment as needed
  • Additionally, the individual will contribute to the implementation of electronic documentation and plasmid inventory management to adequately support research grade rAAV vector manufacturing
This position requires:

  • Knowledge and hands on experience in qPCR, ddPCR and DNA production/characterization
  • Experience with viral vector characterization and quality control assays
  • Experience with DNA sequencing and characterization both in house and outsourced and DNA related software
  • Good written and verbal communication skills and ability to work effectively in a team environment are required


  • Perform quality control assays described above in the job primary duties
  • Medium to large scale plasmid production and plasmid release
  • Perform routine calculations and maintain appropriate records
  • Prepare laboratory reagents/raw materials for release assays and DNA preparation
  • Maintain lab equipment and records
  • Maintain laboratory safety procedures and compliance records
  • Accountable to the research vector core leader
  • Maintain effective communication with therapeutic area scientists on vector release and plasmid production

Education and Experience Requirements:

  • B.S/M.S in Molecular Biology, Biochemistry, Chemical Engineering, or a related discipline
  • Generally, has > 3 years of relevant working experience
  • Experience with qPCR, ddPCR or relevant analytical assay is a plus
  • Proven ability to work independently and effectively plan and organize work activities and prioritize task completion to meet schedules and deadlines
  • Strict attention to details. Excellent organizational and multi-tasking skills are required
  • Excellent personal communication skills

Key Skills, Abilities, and Competencies:

  • Strong organizational skills.
  • Excellent writing and verbal communication skills.
  • Independently motivated, detail oriented and good problem-solving ability.
  • Highly adaptable and comfortable in an environment with dynamic priority demands
  • Ability to multitask.
  • Ability to analyze and troubleshoot experiments.
  • Ability to work independently and as part of a team after initial training

Complexity and Problem Solving:

  • Demonstrate independent problem-solving skills of technical issues.
  • Develop solutions to a variety of moderate to complex problems; discuss with supervisor and refer to policies/practices for guidance.
  • Improve current standard practices when needed.
  • Must be comfortable in the fast-paced, biotech start-up environment and interact with multi-disciplinary research teams at Spark.
  • Exercise judgment within defined procedures and practices to determine appropriate action.

Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.

Nearest Major Market: Philadelphia

Join the Spark Team

We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable - until now.

We don't follow footsteps. We create the path.


At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.

Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.

Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.

Find Us
3737 Market Street
Suite 1300
United States

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