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NGS Scientist

Spark Therapeutics
Philadelphia, Pennsylvania. Hybrid and remote options available for select opportunities.
Salary not provided
Closing date
May 27, 2022

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Health Sciences, Drug Development
Job Type
Staff Scientist
Organization Type
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Job Details

Primary Duties

The NGS Scientist will utilize next-generation sequencing technologies, prepare NGS libraries and perform high throughput sequencing in the NGS core to support Spark Therapeutics pre-clinical pipeline and platforms. Primary duties include:

  • Work closely with the research groups to identify unmet needs and opportunities to utilize NGS technologies to facilitate research projects, such as recombinant AAV vector quality control, vector optimization and screening using DNA barcoding, omics profiling through genome sequencing, transcriptome sequencing and epigenetic sequencing.
  • Collaborate with end users to design experiments, prepare libraries and conduct high throughput sequencing to support various research projects and advance departmental goals
  • Develop, optimize and validate NGS library preparation and sequencing methodologies based on specific needs and ad hoc requests from research groups.
  • Establish quality control metrics, implement quality control measures for NGS workflows in the core. Troubleshoot and ensure accuracy and precision of data generated in the NGS core.
  • Interface cross-functionally with other teams in the company such as the Technology Automation team and the Data Science team to automate library preparation protocols and ensure smooth data flow to improve operational efficiency and robustness of the NGS core.

Job Function and Description

-Routine NGS operations from sample receiving, nucleic acid extraction, library construction, data generation and quality control to ensure high level of quality and reliability of generated results. Maintain laboratory equipment.

-Develop new protocols and optimize existing protocols to meet clients’ ad hoc needs. Improve operational efficiency of the core through automation and innovation. Troubleshoot sequencing experiments as needed.

-Research literatures. Author SOPs and reports. Contribute to interpretation of the data as needed.

-Provide support to the Research Teams, engage in cross-functional communications with different departments.

Education and Experience Requirements

  • PhD in a relevant scientific discipline such as biological sciences, biochemistry, or analytical chemistry. Or Master or bachelor’s degree in a relevant scientific discipline with 3 years or more of related experience within an established laboratory.
  • Solid molecular biology background and deep understanding of underlying principles for molecular biology procedures.
  • Proficient in general molecular biology techniques, such as nucleic acid extraction, molecular cloning, PCR, and next generation sequencing (NGS).
  • Experience in NGS library preparation such as WGS, targeted DNA sequencing, RNA-Seq, ATAC-seq, ChIP-seq, etc.
  • Single-cell sequencing experience preferred.
  • Direct experience with Illumina sequencing platform and familiarity with additional platforms (Oxford Nanopore, PacBio) preferred.

Key Skills, Abilities, and Competencies

  • Highly organized, detail-oriented, goal-driven.
  • Critical thinking skills and continuous improvement mindset
  • Ability to manage timelines and quality of work using strong organizational, communication, and interpersonal skills.
  • Work successfully in a cross-functional project team
  • Proactive and collaborative mindset
Complexity and Problem Solving

  • Design and perform highly complex assays to support departmental objectives
  • Develop solutions to complex problems which require the regular use of ingenuity and innovation.
  • Ensures solutions are consistent with organization objectives
Internal and External Contacts

The incumbent will collaborate with therapeutic area leads/scientists, data science, and other stakeholders involved in experiment design, implementation, and data analysis.

Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.

Nearest Major Market: Philadelphia

Join the Spark Team

We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable - until now.

We don't follow footsteps. We create the path.


At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.

Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.

Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.

Find Us
3737 Market Street
Suite 1300
United States
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