Research Associate (Molecular Biology)

Spark Therapeutics
Philadelphia, Pennsylvania. Hybrid and remote options available for select opportunities.
Salary not provided
Closing date
Jun 5, 2022

Job Details

The Ocular Research group of Spark Therapeutics is seeking a highly motivated Research Associate to develop novel gene therapies for ocular diseases. The successful candidate will play a major role in helping design the best possible vector elements for gene transfer to the retina. He/she will help building DNA constructs, test plasmid and vector functionality, quantify gene expression and tissue specificity in efforts to develop lead candidates.

Primary responsibilities:

    Under the guidance of Ocular scientists, design and execute DNA cloning strategies to construct novel gene cassettes to achieve optimal expression in the target tissue and/or specific cell type. In collaboration with Ocular scientists, test engineered AAV vectors in animal models to quantify gene expression levels, tissue specificity, and/or function using a variety of routine assays (eg. DNA/RNA extraction, ELISA, qPCR, western blot, flow cytometry, immunohistochemistry, etc.) Participate in sample management activities such as receiving, processing, storage and shipping preclinical samples. Accurately document assay results and analyze data using an electronic lab notebook, technical documents, and other reporting formats. Contribute to a collaborative, creative and rigorous culture of scientific discovery


Plan, prepare and perform experiments on the bench to support the Ocular Platform research projects. Assist technical staff in the performance of laboratory experiments as needed.

Analyze results, prepare technical reports, and accurately document work in an electronic lab notebook. Prepare Powerpoint presentations of results as needed. Communicate results and research progress during team meetings as needed.

Participate in group meetings, scientific discussions, and provide support to more junior staff, as needed.


  • BA/BA in Biological sciences required, Master’s in a relevant field preferred.
  • 3-5 years of laboratory experience.
  • Broad knowledge of basic laboratory techniques and equipment.
  • Proven hands-on experience with cell culture and biological assays, such as but not limited to cell-based assays, ELISA, and Western analysis. Experience with WES/Jess technology is a plus.
  • Proven hands-on experience with standard molecular biology techniques, including nucleic acid isolation, PCR, qPCR, DNA cloning.
  • Experience with immunohistochemistry/FISH experience is a plus.
  • Experience with AAV vectors is a plus.
  • Experience with animal handling is a plus.


Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.

Nearest Major Market: Philadelphia

Join the Spark Team

We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable - until now.

We don't follow footsteps. We create the path.


At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.

Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.

Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.

Find Us
3737 Market Street
Suite 1300
United States

Get job alerts

Create a job alert and receive personalized job recommendations straight to your inbox.

Create alert

Similar jobs