A postdoctoral fellow position in the Marasco lab (https://marascolab.dana-farber.org/) in the Department of Cancer Immunology and Virology is available to join our human antibody discovery and chimeric antigen receptor (CAR) T-cell cancer therapy programs. This fellow will lead our efforts in developing immune restoring (IR) CAR T cells and examining the combinatorial effect of CAR T with expression of the immune modulator antibodies on inhibiting tumor growth in vitro and in vivo in ovarian cancer (OvCA). Working with a team of dedicated research staff including computational, cellular, molecular and structural biologists and virologists, this fellow will be responsible for designing and executing in vitro and in vivo experiments for their designated disease area.
The postdoctoral fellow will generate cancer target specific CAR constructs, create CAR T-cells, carry out in vitro assays to test and optimize their biological activities, including generating appropriate tumor target cell lines, optimizing CAR T proliferation conditions, setting up in vitro target cell killing assays, characterizing CAR T cells’ responses to stimulation by target cells such as degranulation, cytokine production, cellular phosphorylation levels of intracellular signaling domains and activation induced cell death. The fellow is expected to set up appropriate tumor model(s) in humanized mice and perform in vivo experiments to evaluate therapeutic potential of specific IR CAR T-cells for the intended cancer target(s). In addition to monitoring tumor growth and metastases, the fellow will assess the activity of CAR T cells (proliferation, exhaustion etc.), and analyze tumor microenvironment by transcriptional profiling using scRNAseq and other molecular means as well as multiparameter flow cytometry/immunohistochemistry. While we are not a computational biology laboratory, support for scRNAseq will be provided but a candidate should become familiar with this field. The candidate must be a self-starter and highly motivated team player. The fellow is expected to work independently, well versed in the related literature, and contribute/lead intellectually to their research program. The team member will also be responsible for providing regular written and oral presentations. The successful candidate must demonstrate excellent writing and verbal communications skills.
Ph.D. degree with documented research experience in molecular biology and immunology, especially in antibody discovery and/or CAR T cell therapy. Experiences in cancer animal model and relevant molecular, biochemical and immunological techniques are preferred. We are particular interested in the candidates with prior research experience in the area of cellular immunology, gene-editing, and/or specializing in mouse surgery and establishing human immune system reconstructed mouse models. The individual is also expected to be able to parallel processing (good time management skills), well organized, and pay careful attention to experimental details. While supervisory experience will be a plus, the candidate should expect to be fully involved in hands-on bench work.
Works in a busy academic research laboratory. Note that nights and weekends may be required to meet research needs.
For more information and to apply, please visit: https://careers.dana-farber.org/research-fellow-marasco-laboratory-1
Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer, HIV/AIDS and emerging infectious diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.
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