Research Assistant II - Dr. Sachet Shukla's Laboratory

University of Texas MD Anderson Cancer Center
Houston, Texas
Closing date
Oct 10, 2022

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Health Sciences
Organization Type
The Shukla lab is a highly translational group focused on antigen discovery and development of personalized immunotherapies. The primary purpose of the Research Assistant II position is to provide technical assistance in carrying out various research projects while following standard operating procedures for the laboratory.

  • Caring: By our words and actions we demonstrate caring toward everyone.
  • Integrity: We work together with professionalism to merit the trust of our colleagues and those we serve in all that we do.
  • Discovery: We embrace creativity and seek new knowledge from diverse perspectives.
  • Safety: We provide a safe environment - physically and psychologically - for our patients, for our colleagues and for our community.
  • Stewardship: We protect and preserve our institutional reputation and the precious resources - people, time, financial and environmental - entrusted to us.

Cell Culture, MacroMolecules, Hazardous/Radioactive Substrates & Patient Tissues
** Maintain stock cancer cell lines for cell culture, to include: growing, expanding, and freezing utilizing sterile techniques. ** Ability to isolate and assay macromolecules such as DNA, RNA, and proteins. Successful completion of these assays may require knowledge of molecular biology techniques. **Ability for perform standard immunologic (e.g. primary T cell isolation, flow cytometry, ELISA, intracellular cytokine assays) and molecular biology (e.g. PCR, cloning, Westerns) techniques. **Ability to do immunoprecipation **Work with standard in vitro transcription and translation kits ** Acquire training to run PacBio, MinION, 10x machines. Some procedures will require the use of hazardous and/or radioactive substrates. Must maintain appropriate environmental surveys for radioactivity and other hazardous materials. and maintain records of hazardous chemicals and reagents as required. **Handle patient tissues, with familiarity of safety procedures.

Record Keeping & Professional Literature
** Manually and promptly record research data, analyzes data, prepares graphs, charts, and/or tables for publications plus have knowledge of word processing programs and spreadsheets. **Discusses results from each assay to ensure accuracy and comparability with previous results of similar assays and reconciles discrepancies as soon as possible. **Reads and discusses selected literature with supervisor and other laboratory personnel.

Assisting & Teaching Others and Lab Management Responsibilities
** Assists other laboratory personnel with animal experiments as necessary, to include: injection of cancer cells, assessing tumor size, and harvesting tumors. ** Helps laboratory manager teach techniques to graduate students, surgical fellows, and other research personnel. **Responsible for partial lab management duties such as stocking supplies, maintaining equipment, and ensuring lab productivity and workflow.

Analytical Thinking - Gather relevant information systematically; break down problems into simple components; make sound decisions.

Innovative Thinking - Approach problems with curiosity and open-mindedness; offer new ideas, solutions and/or options.

Technical/Functional Expertise - Demonstrate technical proficiency required to do the job; possess up-to-date knowledge in the profession; provide technical expertise to others.

Service Orientation
Provide service to our stakeholders, including patients, caregivers, colleagues and each other, in a safe, courteous, accountable, efficient and innovative manner to include:
• Promoting inclusiveness and collegiality that demonstrates respect and professionalism to our stakeholders at all times
• Modeling safe, ethical behavior that mitigates risk to the institution through sound business practices, and adherence to MD Anderson's Standards of Conduct, institutional policies and procedures
• Responding to requests in a timely manner while proactively communicating expectations for procedures, service arrival, or project deliverables to stakeholders
• Using the steps to HEAL (Hear, Empathize, Address and Learn) the relationship when service recovery is needed


Required: Bachelor's degree with major course works in one of the basic sciences.

Preferred: Master's Level Degree in immunology, cancer biology, biotechnology or related scientific discipline.


Required: Two years experience in scientific or experimental research work. No experience required with preferred degree.

Preferred: Experience with DNA and RNA extraction, purification and isolation techniques.

It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law.

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