Postdoctoral Fellow Opportunity in Cardiovascular Research
Department Name/Job Location:
This opportunity is at Washington University School of Medicine in the Department of Internal Medicine – Division of Cardiology and located at CSRB – North Tower Addition on the Medical School Campus.
This opportunity is full-time. Hours are generally normal business hours but may vary due to the timing of experiments and other research activity deadlines.
The interest of this academic laboratory (PI: Babak Razani) is understanding the mechanisms of atherosclerosis, diabetes, obesity, and fatty liver disease with special emphasis on the roles of macrophages. We and others have shown that perturbations in cellular processes involving mTOR and nutrient sensing, autophagy and lysosomes, and inflammatory signaling including inflammasomes play a critical role in macrophage dysfunction and subsequent cardiometabolic disease. Some of our relevant primary papers include (Sergin, et al Science Signaling 2016 and Sergin, et al Nature Communications 2017; Evans, et al Science Signaling 2019; Zhang, et al Nature Metabolism 2020; Jeong, et al Autophagy 2021). The primary focus of the lab will be to understand the mechanisms by which these cellular processes affect metabolism, determine if their manipulation ameliorates metabolic disease, and translate our findings to develop novel therapeutics in human coronary artery disease, obesity, and insulin resistance.
These questions are being studied using a combination of molecular and cell biology approaches in mouse primary cells/cell-lines, mouse models, human monocytes/monocyte-derived macrophages, induced pluripotent stem cell (iPSC)-derived macrophages, and circulating monocytes and tissue macrophages from humans enrolled in clinical studies. Additionally, there is a recent focus for the lab on “omics” approaches to macrophage phenotyping and using CRISPR/Cas9 techniques to target newly identified genes for subsequent cellular and organismal phenotyping.
Primary Duties & Responsibilities:
- Collaborates with the PI on the design, conduct and reporting of research projects.
- Assists with grant preparation and reporting. May submit grant proposals with the approval of the PI, Department Head and Dean.
- Prepares and submits papers on research.
- Develops hypotheses to be tested and ways of testing it.
- Complies with established safety procedures and maintaining required documentation on laboratory and specimen conditions.
- Manages research projects with other institutions and investigators.
- Candidates with extensive prior experience in cell, molecular biology, immunology, metabolism, or related fields with a Ph.D. degree and four years of experience in research lab operations, or an equivalent combination of education and experience equaling nine years are encouraged to apply.
- Mammalian cell culture.
- Basic Microbiology skills.
- Experience with microscopy especially confocal/fluorescence microscopy.
- Proficiency in basic molecular biology techniques including DNA, RNA, protein isolation and purification, PCR, cloning, reverse transcription PCR, immunoblot, ELISA.
- Experience with immunocyto- and histo-chemistry, experience with frozen/paraffin tissue.
- Experience with flow cytometry and immunology.
- Ability to perform small animal procedures.
- Experience with CRISPR/Cas9 targeting strategies including in vitro screens.
- Experience with RNAseq and other “omics” strategies with related bioinformatics tools to handle large data sets from RNA seq, scRNA seq, proteomics, and lipidomics experiments.
Salary will follow NIH guidelines and will be adjusted by achievement.
This opportunity is eligible for full-time benefits. Please visit our website at http://hr.wustl.edu to view a summary of benefits.
Applicant Special Instructions:
Please contact Dr. Babak Razani at firstname.lastname@example.org or at 314/362-3688. Candidates must provide a current CV and 2 to 3 references that can be contacted directly by phone and/or email.