Research Assistant I- Cell Culture

Summary

The Mancini Lab is seeking to recruit a full time Research Assistant I (RA-I) to support cell culture, imaging-based assays and advanced microscopy experiments designed to investigate the relationship between changes in nuclear shape/stiffness and gene expression based upon single cell analysis via imaging approaches. The position would principally involve carrying out experimental work using previously developed protocols, optimization of novel assay protocols with support from senior lab personnel, and imaging on the available instruments within the lab-associated, Integrated Microscopy Core. Teamwork, flexibility, multi-tasking, optimal record keeping and data sharing, strong communication (written/spoken) and interpersonal skills are all essential to a successful application. Prior experience with microscopy techniques is preferred; however, extensive training will be provided.

Mike Mancini lab interests revolve around single cell analysis of gene expression, primarily through native or engineered estrogen receptor model systems. Model systems are designed for use with quantitative, high content analysis using a wide range of imaging platforms, ranging from high throughput microscopy to multiple super resolution platforms (SIM, STED, STORM) followed by image analysis, data mining and classifications approaches, including deep learning and neural networks.

Job Duties

• Operate instrumentation required for specific projects (training will be provided as needed).
• Perform routine cell culture in support of experiments.
• Document experimental procedures in precise details.
• Collect and compile imaging data.
• Prepare reagents and solutions, and perform experimental work as needed.
• Troubleshoot problematic results.
• Directly interact with lab and Core personnel.
• Maintain core laboratory organization, monitor lab reagents and do ordering.
• Optimize novel assays and protocols with support from senior lab personnel.

Minimum Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Two years of relevant experience.

Preferred Qualifications

• Master's degree in a related field.
• Applicants should have familiarity with laboratory techniques, and previous professional experience related to working in a biological laboratory.
• Cell culture and basic biochem/molecular biology knowhow is essential.
• Immunhistochemistry and immunofluoresence a plus.

Baylor College of Medicine requires employees to be fully vaccinated -subject to approved exemptions-against vaccine-preventable diseases including, but not limited to, COVID-19 and influenza.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

10213

CA; SN