Senior Histologist

Spark Therapeutics
Philadelphia, Pennsylvania
Salary not provided
Closing date
Feb 5, 2022

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Life Sciences, Biotechnology
Organization Type

Job Details

Primary Duties:

This individual will be responsible for processing animal tissue samples for digital histopathology and maintaining the daily operations in the histology core at Spark Therapeutics. They will be expected to work with scientists, bioimagers and pathologists to create and optimize histological assays. Candidates that have previous experience in grossing and processing ocular and central nervous system tissues from a variety of animal models are preferred.


  • All phases of tissue preparation, including fresh or in-process specimens (paraffin and frozen)
  • Routine paraffin and cryo embedding and sectioning, H&E (Hematoxylin & Eosin) staining, special stains technique (histochemistry), immunohistochemistry, immunofluorescence, and in situ hybridization are required.
  • Accountable for examining tissues upon receipt and applying understanding of gross anatomy and study purpose to determine and dissect appropriate representative samples for histology testing and Pathology analysis.
  • Specimen preparation that requires a complex and thorough understanding of tissue structure, as well as a basic understanding of chemistry and physics; and how tissues react under process conditions, including decalcification, rehydration, grossing, fixation, embedding, microtomy, routine staining, special stains (histochemistry), and immunohistochemistry.
  • Develop and drive histology-based assays (through external and internal collaboration) including immunohistochemistry (IHC), immunofluorescence (IF) and in-situ hybridization (ISH). Capable of troubleshooting and identifying artifacts.
  • Able to perform in-house qualitative image analysis across different tissue staining platforms; would be comfortable connecting with bioimagers and data scientists to optimize how the samples are processed for quantitative image analysis.
  • Expert-level written summarization of findings (PowerPoint presentation, research reports etc.) and proficient verbal communications to peers, management, and large groups as projects evolve

This individual will also be responsible for maintenance, operation, qualification/validation of histology equipment, as well as training technicians and peers in histological sample handling, histochemical and immunohistochemical technique, imaging and analysis.


  • Hands-on Tissue processing, staining and image acquisition
  • Develop and validate histology-based biomarker assays
  • Participate in study investigations
  • Communicate findings and provide presentations
  • Review and revise SOPs and/or work instructions
  • Maintain equipment and records
  • Maintain laboratory safety procedures and compliance records
  • Prepare laboratory reagents/materials

Education and Experience Requirements:

  • B.S./M.S. in relevant field with 5+ years pre-clinical histology experience; having HT/HTL (ASCP) certification would be preferred
  • Generally has 3-5 years in ocular or CNS fields
  • Experience performing and troubleshooting singleplex and multiplex IHC, IF, and/or ISH assays
  • Excellent interpersonal and communication skills as well as the ability to work effectively as part of a cross-functional team

Key Skills, Abilities, and Competencies:

  • Strong organizational skills.
  • Excellent writing and verbal communication skills.
  • Independently motivated, detail oriented and good problem solving ability.
  • Ability to multitask.
  • Ability to analyze and troubleshoot experiments.

Complexity and Problem Solving:

  • Demonstrate independent problem solving skills of technical issues; develop solutions to a variety of moderate to complex problems; Discuss with supervisor and refer to policies/practices for guidance.
  • Must be comfortable in the fast-paced, biotech start-up environment and interact with multi-disciplinary research teams at Spark.
  • Exercise judgment within defined procedures and practices to determine appropriate action.


Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.

Nearest Major Market: Philadelphia

Join the Spark Team

We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable - until now.

We don't follow footsteps. We create the path.


At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.

Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.

Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.

Find Us
3737 Market Street
Suite 1300
United States

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