Pfizer

Senior Scientist, Hydridoma, BioMedicine Design

Employer
Pfizer
Location
La Jolla, California
Salary
Competitive
Posted
November 25 2021
Ref
4829911
Position Type
Full Time
Organization Type
Pharma
Job Type
Senior Scientist
Role Summary

BioMedicine Design of Pfizer Inc. is seeking a highly motivated Hybridoma Senior Scientist in La Jolla, CA. This Sr. Scientist level position will directly contribute towards our efforts for discovery of monoclonal antibodies using hybridoma technologies. The candidate will support different antibody discovery programs while utilizing a broad range of immunology, molecular biology and cell biology-based techniques. The duties of the job include experimental design and execution, analysis and interpretation of data, and presentation of results to the research team. This position will be exposed to the state of art technology in single cell analysis, NGS sequencing and the advanced automation system.

Role Responsibilities

Primary responsibilities include but not limited to:
  • Execute on all stages of experiments to generate monoclonal antibodies through hybridoma technology, including animal immunizations, serum titer tracking and suspension splenocytes preparation, hybridoma fusion and screening, and candidate identification.
  • Sequence the VH and VL of hybridoma hits, analyze antibody diversity, and choose representative clones for recombinant production.
  • Regular cell culture and maintenance. Transient expression of antibodies in mammalian cells. Small scale purification of recombinant antibodies.
  • Interface with external CROs to identify need for reagents, define timelines and scheduling to meet project goals.
  • Trouble-shoot and optimize protocols and propose creative solutions to improve efficiency to existing processes.
  • Interpret and summarize experimental data. Participate in the evaluation of new methods or technologies.
  • Maintain detailed records of all experiments in ELN and using in-house databases and tracking systems in a detailed and descriptive manner.


Basic Qualifications
  • MS in biological sciences with over 5 years antibody-related experience in an industrial setup. Knowledge in B cell biology and antibody generation process is essential.
  • Experience with hybridoma technology including hands-on-skills in the performance of fusions as well as downstream screening and characterization activities for generation of monoclonal antibodies in mice and hamster.
  • Successful experience in designing of immunization strategies to elicit potent humoral response in rodents. Familiarity with dosing animals, serum collection and titer test.
  • Extensive experience with multiple antibody screening assays and analysis, such as ELISA and FACS. Good organizational skills, with scientific rigor and critical thinking.


Preferred Qualifications
  • PhD in immunology is highly preferred.
  • Experience with antibody VH/VL sequencing and cloning for hybridoma hits NGS based high throughput sequencing is a plus.
  • Experience in DNA-based immunization is a plus
  • Experience with cell sorting, liquid handling, and automation is desired.


Other Job Details:
  • Last Date to Apply: November 29, 2021
  • Eligible for Relocation Package: Yes
  • Eligible for Employee Referral Bonus: Yes


#LI-PFE

Pfizer requires all U.S. new hires to be fully vaccinated for COVID-19 prior to the first date of employment. As required by applicable law, Pfizer will consider requests for Reasonable Accommodations.

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development

#LI-PFE

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