PD Upstream Scientist Gene Delivery
- Employer
- Spark Therapeutics
- Location
- Philadelphia, Pennsylvania
- Salary
- Salary not provided
- Closing date
- Feb 26, 2022
View more
- Discipline
- Health Sciences, Drug Development
- Job Type
- Staff Scientist
- Organization Type
- All Industry, Biotech
Job Details
- Developing strategies to optimize efficacy and toxicity of non-viral gene delivery approaches.
- Synthesizing, characterizing, and evaluating novel formulations for nucleic acid delivery.
- Performing experiments in the development, optimization, and scale-up of recombinant adeno-associated viral vector (rAAV) production processes in suspension cell culture systems, with a focus on shaker flask, stirred tank bioreactor production at bench and pilot scales.
- Analyzing and interpreting experimental results; suggesting changes or additional experiments to improve the system performance, robustness and/or reproducibility.
- Participating in discussions regarding the troubleshooting of bench and pilot scale production processes and/or investigations; performing support studies as needed.
- Contributing to the evaluation and implementation of new technology development as appropriate.
- Participating in Technology Transfer from process development to manufacturing sites; assisting in training manufacturing staff as needed.
- Executing protocols, assembling data, and drafting appropriate technical documentation, which may include, but is not limited to SOPs and Process Development Reports.
- The successful candidate will be skilled in formulation science, cell culture or engineering principles.
- He/she should have hands on experience in formulation or upstream process development, including familiarity and knowledge of formulation and gene delivery, capability for troubleshooting experiment and/or equipment issues, and ability to work/communicate effectively with fellow team members, as well as with project teams.
- Typically, has a Ph.D. in a relevant scientific discipline (e.g., pharmaceutical sciences, chemical engineering, biological engineering, molecular biology, virology, biochemistry, etc.) or a B.S./M.S. in a relevant scientific discipline with 3+ years of relevant scientific and/or technical experience in the life sciences/Biotech field.
- Knowledge or experience of formulation sciences is essential.
- Adept technical writing skills with experience authoring/reviewing technical documentation (protocols, reports, SOPs, etc.) is preferred.
- Familiarity with gene delivery and/or biomaterials is a plus.
- Strong understanding of the technical and equipment needs in a lab as well as significant hands-on experience with standard molecular and cell biology techniques such as ELISA, qPCR, cell culture, transfection, western blotting, etc. is a plus.
- Familiarity with statistical design/analysis software is a plus.
- Experience and knowledge in general and advanced nanoparticle formulation techniques.
- Experience or knowledge with different characterization methods such as flow cytometry, dynamic light scattering and zeta potential.
- Proven ability to work independently in hands-on laboratory setting, self-motivated to learn and develop new techniques, perform multiple tasks simultaneously, keep accurate records, follow instructions, and comply with company policies.
- Good scientific knowledge of cell and molecular biology, biochemistry, and formulation sciences.
- Capability in experimental design and data analysis, and experience troubleshooting.
- Ability to evaluate, refine, and implement new technology.
- Excellent written and verbal communication skills.
- Planning and organizational skills.
- Demonstrate problem-solving skills when faced with technical issues; capable of working on more than one task and developing solutions for moderately complex problems. Discuss with supervisor and refer to policies/practices for guidance.
- Must be comfortable in the fast-paced, biotech start-up environment and interact with multi-disciplinary Research, PD, and Tech Operations teams at Spark.
- Exercise judgment within defined procedures and practices to determine appropriate action.
- Fellow Upstream PD Team Members to discuss scientific and technical issues.
- Cross-functional Team Members such as: R&D Operations, PD Downstream, PD Analytical, PCO, Technical Operations, Quality Assurance, Quality Control, Regulatory Affairs, Facilities, Materials Management, and Service Personnel/Vendors.
- External Vendors for the evaluation of new technologies, instruments and/or materials.
- External contact development and/or manufacturing organizations.
- Must be able to lift 25 pounds.
- Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule.
- Handling large volumes of cell culture or related materials, such as tens of liters, may be required.
- Although infrequent, some travel may be required.
Please be aware that Spark mandates COVID-19 vaccination of all employees regardless of work location. Accommodations may be made in accordance with applicable law.
Nearest Major Market: Philadelphia
Join the Spark Team
We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable - until now.
We don't follow footsteps. We create the path.
Company
At Spark Therapeutics, a fully integrated, commercial company committed to discovering, developing and delivering gene therapies, we challenge the inevitability of genetic diseases, including blindness, hemophilia, lysosomal storage disorders and neurodegenerative diseases.
Founded in March 2013 as a result of the technology and know-how accumulated over two decades at Children’s Hospital of Philadelphia (CHOP), our investigational therapies have the potential to provide long-lasting effects, dramatically and positively changing the lives of patients with conditions where no, or only palliative, therapies exist. Greater understanding of the human genome and genetic abnormalities have allowed our scientists to tailor investigational therapies to patients suffering from very specific genetic diseases. This approach holds great promise in developing effective treatments to a host of inherited diseases. Our initial focus is on treating orphan diseases.
Headquartered in dynamic Philadelphia, we are a diverse, experienced team united in our goal to break down barriers for people and families affected by genetic diseases. As our name suggests, our investigational, one-time therapies are designed to spark healthy biology, and deliver potentially life-altering transformation for people and families affected by genetic disease. Spark is a member of the Roche Group.
- Website
- http://sparktx.com/
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