Research Scientist II
The James and Rawlings labs at Seattle Children’s Research Institute are hiring motivated scientists to join a rapidly expanding research program focused on B cell engineering. The ultimate goal of the B cell engineering group is to develop novel cell therapies that provide sustained production of therapeutic proteins from gene-edited, adoptively transferred, autologous plasma B cells.
For this position, we are seeking a highly motivated aspiring biologist to participate in projects in the primary B cell engineering and differentiation sub-group. Specifically, the successful candidate will lead experiments focused on the development and assessment of allogeneic human B cells. The candidate will culture and engineer primary B cells to express proteins that prevent an immune response targeting the engineered cells. The resulting cell product will be transferred to an animal model and containing MHC mismatched immune cells. Survival of these cells will be assessed following sacrifice using flow cytometry and ELISA.
The applicant will collaborate with senior scientists and the principal investigators to plan, execute and interpret experiments. The applicant will be required to independently select and apply standardized scientific procedures, contribute to the design and planning of experiments, troubleshoot experiments, make detailed observations, record data and assist in the preparation of reports, papers and presentations. In this role, the research scientist will be involved with both cell biology applications and in rodent experiments.