Senior Scientist, RNA Production

Cambridge, Massachusetts
October 22 2021
Position Type
Full Time
Organization Type
Job Type
Senior Scientist

Pfizer's BioMedicine Design Biotherapeutic Expression and Purification group (BEP) is seeking a candidate to join their team. The candidate will play an essential role in a high-energy RNA production research laboratory. The production group is responsible for non-GMP material generation, supporting the various Pfizer research units and their pre-clinical therapeutic in-vitro and in-vivo needs. The candidate will be responsible for in-vitro transcription (IVT), purification and characterization of mRNA products through the use of state-of-the-art technologies and equipment. The candidate will contribute to the group wide deliverables and be required to work on several projects simultaneously presenting results and updates at group meetings.

  • Develop efficient IVT & purification protocols for novel RNA therapeutic candidates
  • Provide highly-purified candidates to discovery project teams
  • Drive technology innovation; design, suggest and/or implement strategies to increase throughput and/or efficiency
  • Work closely with peers to meet deadlines and project goals
  • Effectively troubleshoot technical issues
  • Independently perform experiments using a wide-range of enzyme-driven reactions, chromatographic, analytical techniques
  • Maintain high quality lab notebook and demonstrate effective record-keeping
  • Present work to the BEP group and project teams
  • Knowledge of chemical reactions
  • Ability to multitask
  • Knowledgeable and experienced in preparative and analytical chromatography methods
  • mass spectrometry, ELISA, SDS-PAGE gel, endotoxin assay, 260/280 measurements, agarose gel, fragment analyzer

  • PhD in Biochemistry, Biology, Biomedical Sciences or a related discipline with 1-3 years of relevant experience along with an understanding of RNA therapeutic production
  • Understanding and experience with IVT, RNA capping & purification
  • Enzymatic reactions
  • DNA/RNA handling/precipitation
  • Experience with affinity, SEC, IEX, HIC and various chromatography techniques
  • Agarose gel/fragment analyzer
  • Experience or knowledge in the operation of AKTA (LC) and Agilent (HPLC) chromatography systems
  • Strong communication skills, written and oral
  • Can execute experiments independently
  • Works well as part of a team
  • Creative/flexible thinking

Other Job Details:
  • Last Day to Apply: October 26, 2021
  • Eligible for Employee Referral Bonus
  • #LI-PFE

Pfizer requires all U.S. new hires to be fully vaccinated for COVID-19 prior to the first date of employment. As required by applicable law, Pfizer will consider requests for Reasonable Accommodations.

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development


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