Pfizer

Technical Operations II, Plasmid DNA

Employer
Pfizer
Location
Cambridge, Massachusetts
Salary
Competitive
Posted
July 16 2021
Ref
4817066
Position Type
Full Time
Organization Type
Pharma
Why Patients Need You

Pfizer's purpose is to deliver breakthroughs that change patients' lives. Research and Development is at the heart of fulfilling Pfizer's purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best in class medicines to patients around the world.

What You Will Achieve

Pfizer's BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory. The group supports non-GMP transient protein lead production across Pfizer's diverse biotherapeutic discovery portfolio to enable Pfizer's research units and their pre-clinical therapeutic in-vitro and in-vivo needs. The BEP DNA production and protein expression group is looking for a laboratory technician to provide DNA production support for the protein expression and purification activities in BEP. The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis. The successful candidate will grow bacterial cultures and perform large-scale DNA production activities following standardized protocols. The candidate will be a key contributor to the scientific team responsible for protein expression and purification.

How You Will Achieve It

  • Production of plasmid DNA and maintenance of plasmid library to support transient recombinant protein expression including bacterial cultures and automated DNA production
  • Incorporate and use automated instrumentation for plasmid purification
  • Conduct routine DNA characterization and quality control testing including gel electrophoresis and sample preparation for sequencing analysis.
  • Maintain a record of daily work in an organized electronic lab notebook and use an online database to keep inventories and manage samples and key documentation
  • Maintain a rotating stock of supplies for DNA production activities
  • Interface with expression scientists on the team as well as scientists in the department submitting protein production requests
  • Effectively communicate with the team to meet strict schedules and deadline


Qualifications

Must-Have

  • B.Sc in biological/life sciences or Associates / Certificate in Biotechnology, or High School diploma with at least 2 years of relevant laboratory research school
  • Previous microbiology experience
  • Must have good practical skills in a laboratory setting such as keeping an organized laboratory notebook and maintaining laboratory cleanliness to minimize contamination
  • Ability to multitask and keep an organized record of daily work in an electronic lab notebook
  • Must be able to follow detailed protocols and weekly schedules
  • Must have the ability to concentrate while doing repetitive tasks
  • Must follow laboratory safety rules


Nice-to-Have

  • Experience working with bacterial cell lines and cell culture
  • Proficiency in nucleic acid purification and quantitative analysis techniques


PHYSICAL/MENTAL REQUIREMENTS

Lifting, sitting, standing, walking, bending, ability to perform mathematical calculations and ability to perform complex data analysis

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development

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