Research Associate II - Protein Biochemistry

Duarte, California
June 11 2021
Position Type
Full Time
Organization Type
About City of Hope

City of Hope, an innovative biomedical research, treatment and educational institution with over 6000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy.

Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research. City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation's leading cancer centers that develops and institutes standards of care for cancer treatment.

Position Summary:

Under general supervision of the Principal Investigator, or designee, the Research Associate II (RAII) undertakes research projects in collaboration with academic supervisor, making innovative contributions pertaining to laboratory procedures which may not change the course of an investigation but do indicate significant independent contribution beyond the RA I level. RAII typically consults with the academic supervisor on the scope and plan of approach to a research project; reads abstracts and articles; organize and plan techniques; plan and assign the work of at least one lab staff advising on problems they are unable to solve; contribute original ideas to the laboratory research; standardize new techniques and train staff on these techniques; perform specialized procedures and prepare written reports of laboratory experimental methods.

The Kalkum lab is recruiting a Research Associate II to assist on lab research activities, in particular, to perform molecular cloning, expression and purification of recombinant proteins from E. coli or other expression systems, using a range of techniques, including metal affinity chromatography, size exclusion FPLC, refolding approaches and preparative dialysis.

Experience in molecular biology, molecular cloning, DNA isolation, plasmid preparations, SDS PAGE analysis, culture of microorganisms, protein purification is advised. If not already experienced, we offer the opportunity to learn mass spectrometric analysis of proteins, peptides, and other biomolecules.

Key Responsibilities include:
  • Performs a variety of routine and non-routine laboratory research experiments. Records and validates results for accuracy and completeness.
  • Troubleshoots procedures if experiment results are unexpected or unsatisfactory. Recommends alternative procedures.
  • Responsible for accurately recording work performed including results and methodology. Calculates, compiles and analyzes data obtained from work performed.
  • Independently evaluates research data and initiates alternative approaches to improve the quality of results by providing input to overall research design. Trains and collaborates with lab staff in specialized procedures, equipment and theoretical concepts behind unique tests.
  • May contribute to the preparation of manuscripts by describing methods and specialized techniques. Keeps abreast of new and improved techniques.
  • Ensures that all laboratory activities are carried out in compliance with each procedure(s) by utilizing safety procedures and guidelines in handling, storage and disposal of bio-hazardous, infectious and toxic material.

Basic education, experience and skills required for consideration:
  • Bachelor's or Master's degree in Biology, Biochemistry, Immunology or related disciple with a background in protein biochemistry and microbiology.
  • 2 years of related research/laboratory experience with a Bachelor's degree or 1 year of year of related research/laboratory experience with a Master's degree.
  • Experience in molecular biology, molecular cloning, DNA isolation, plasmid preparations, SDS PAGE analysis, culture of microorganisms, protein purification is ideal.
  • Highly motivated, responsible, reliable, smart-working, and eventually become a specialist in the area protein biochemistry and diagnostic assay development.

Additional Information:
  • For more information on Dr. Markus Kalkum's research please visit, here .

City of Hope is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, or status as a qualified individual with disability.

  • Posting Date: Feb 12, 2021
  • Job Field: Research
  • Employee Status: Regular
  • Shift: Day Job

Similar jobs

Similar jobs