Postdoctoral Scientist (m/f/d) Collaborative Research Unit

The Cellular Biophysics and Translational Cardiology Section, together with the Core Facility Proteomics, offers a position for a:

Postdoctoral Scientist (m/f/d)

Collaborative Research Unit CRC1002

limited until 06/24, full time | salary according to TV-L

The University Medical Center Göttingen is a tertiary care center and offers great development potential. Its 7,900 employees work in over 65 departments and facilities to provide top-quality patient care, excellent research and modern teaching. Göttingen, “City of Science”, located near the center of Germany, the University Medical Center Göttingen is embedded in the city’s attractive network of scientific research facilities.

The Cellular Biophysics and Translational Cardiology Section at the Heart Research Center, together with the Core Facility Proteomics, offers a research position for a highly motivated Postdoctoral Scientist. The intended project “Proteomic analysis of local subcellular calcium cycling machines in cardiomyocytes” is part of the Collaborative Research Center CRC1002, a competitive and highly interdisciplinary large-scale collaborative multi-PI project funded by the DFG (https://www.dfg.de/en/funded_projects/current_projects_

programmes/list/projectdetails/index.jsp?id=193793266&sort=nr_asc&prg=SFB).

Beat-to-beat cardiomyocyte function depends on protein interactions in thousands of subcellular nanodomains complexes between the sarcolemmal surface membrane and the sarcoendoplasmatic reticulum that jointly control local metabolic and signaling functions. The local proteomic clustering and functional interactions of the RyR2 Ca2+ release channel and SERCA2a Ca2+-ATPase in high MW complexes in rodent and human cardiomyocytes and their endogenous regulation by a variety of micropeptides provides in-depth innovative cell biology and therapeutic development questions. Recent discoveries identified the complexity of protein/protein interactions of phospholamban and its human mutation proteoforms in this context as the basis for therapeutic targeting towards new models that conceptually underpin precision medicine.

The goal of the project is to establish the underlying mechanism and targeting of the subcellular protein interactions using quantitative biochemistry, mass spectrometry and high-resolution imaging approaches. Of particular interest are human phospholamban mutations and the resulting molecular loss-of-function mechanisms affecting cardiomyocytes. The scientist will work with human iPSC cell models, rodent primary cardiomyocytes and tissues and develop therapeutic interventions to manipulate cardiac disease mechanims in a highly interdisciplinary setting. As part of the project, he / she will employ mainly protein biochemistry and mass spectrometry, as well as confirmatory superresolution microscopy techniques.

The candidate will be located at the Heart Research Center Göttingen and participate in the campus-wide CRC1002 and Excellence Cluster MBExC 2067 and Hertha Sponer College activities.

What you will be doing:

• Applying and optimizing state-of-the-art protein-protein interaction biochemistry and mass spectrometry protocols, high-resolution subcellular imaging and analysis, analyzing mass spectrometry data in samples ranging from cultured cells to cardiac tissue samples.
• Building and applying your collaborative networking and scientific portfolio through associated cardiovascular research projects in the CRC1002.
• Contributing through teaching and collaboration to the growing Campus-wide MBExC and Hertha Sponer College activities at the supervisor level (www.mbexc.de).
• Who you are: A highly motivated postdoctoral scientist with a strong ambition to become independent and excel in academia or industry. You like to communicate openly with scientists and enjoy working in a highly interdisciplinary, multicultural research environment towards your competitive goals.

Your background ranked according to relevance:

• Protein biochemistry; cell and molecular biology; proteomics and mass spectrometry. Experience in high-resolution imaging would be advantageous but is not mandatory.

Mandatory training:

• Protein biochemistry, protein and peptide separation, mutagenesis, proteinprotein interaction analysis, mass spectrometry, multiplexed data analysis, manuscript writing.

What our group offers:

• An exciting collaborative research environment with state-of-the-art methods and equipment at the University Medical Center Göttingen.
• Our group has established advanced techniques in mass spectrometry-based proteomics and high-resolution imaging. Our experience in methods teaching in interdisciplinary teams will provide you with the knowledge and skills you need to carry out your experiments.
• A competitive scientific project embedded in a research center with plenty of opportunities to interact and learn from researchers from various disciplines.
• Scientific retreats and conferences are part of your work and provide opportunities for scientific networking and collaborations.
• Direct interaction with an active proteomics and imaging research community in Göttingen.

The University Medical Center Göttingen is committed to professional equality. We therefore seek to increase the proportion of under-represented genders. Applicants with disabilities and equal qualifications will be given preferential treatment.

We look forward to receiving your application by June 20^th, 2021:

University Medical Center Göttingen

Clinic for Cardiology and Pneumology

Prof. Dr. S. Lehnart

D-37099 Göttingen

Tel.: +49-551-3963630

Fax: +49-551-3963713

E-Mail: slehnart@med.uni-goettingen.de

Web: https://herzzentrum.umg.eu/forschung/arbeitsgruppen/zellulaere-biophysik-und-translationale-kardiologie/

Please send your application via E-Mail in PDF-format or via mail in copy and not in folders.

Travel and application fees cannot be refunded or transferred.