Senior Scientist, Antibody Discovery-Phage Display

La Jolla, California
May 12 2021
Position Type
Full Time
Organization Type
Job Type
Senior Scientist

The Cancer Immunology Discovery (CID) group in La Jolla, CA is focused on the generation of large molecule immunotherapeutics that promote anti-tumor activity or counter immunosuppression in the tumor microenvironment. CID's efforts are supported by a world class antibody generation program, and a commitment to effective biotherapeutic design that stresses both ingenuity and simplicity. Our team takes a holistic approach to understanding the interplay of tumor, stromal, and immune cells in the tumor microenvironment, striving to define the axes of intercellular communication that could be leveraged to generate novel therapeutics.

We are seeking a qualified candidate to join us at a Senior Scientist level to contribute to our antibody discovery and characterization activities for the identification of novel biotherapeutics. The successful candidate will work closely with individuals in antibody production, protein engineering and immunology/oncology groups to innovate and develop the next-generation of protein therapeutics for cancer immunotherapy.

The candidate should have expertise in medium to high throughput large molecule screening using phage display, cell culture, molecular cloning as well as protein production, purification and characterization.

  • Responsible for employing display techniques for discovery of antibodies with novel function for cancer-relevant targets.
  • Establish and implement phage display system for protein and cell-based panning
  • Design and build recombinant proteins for screening.
  • Design and execute screening assays on both protein and cells.
  • Reformat recombinant antibodies into other isotypes, species and conformations (bispecific, scFv, tetravalent, etc.)
  • Purify and analyze proteins and antibodies for form and function using both biophysical characterization.
  • Efficiently analyze and report data, with recommendations for next steps.
  • Present research results and discuss methods at weekly and/or monthly group meetings.

  • Ph.D. in immunology, molecular biology, cell biology or related discipline with a minimum of two years post graduate experience, or M.S. with 10 years experience.
  • Proven track record of antibody discovery using phage display.
  • Skilled in library generation and manipulation.
  • Expertise in large molecule screening assay development for binding and functional activity.
  • Hands-on experience with molecular biology techniques: DNA/RNA isolation, PCR, RT-PCR, cloning, vector design, DNA sequencing.
  • Protein production by transient expression.
  • Experience with protein/antibody purification, protein modification and biophysical characterization.
  • Strong background in cell culture and in vitro assays.

  • Hands-on experience with alternate antibody formats.
  • Experience with up to three-color flow cytometric screening and data analysis.
  • Capable with methods for antibody affinity measurement and analysis (SPR or BLI)
  • Experience with instruments such as GE AKTA Pure/Avant FPLC, fortebio Octet, Biacore T200 or 8K.
  • Robotic liquid handler experience.
  • Previous industry experience is strongly preferred.

  • Periodic overtime and weekend work

  • Eligible for Employee Referral Bonus
  • Eligible for Relocation Package


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Research and Development