Research Assistant II - Molecular Techniques

The University of Texas MD Anderson Cancer Center in Houston is one of the world's most respected centers focused on cancer patient care, research, education and prevention. It was named the nation's No. 1 hospital for cancer care in U.S. News & World Report's 2019 rankings. It is one of the nation's original three comprehensive cancer centers designated by the National Cancer Institute.

KEY FUNCTIONS

Molecular Techniques & Single Cell Sequencing

• Isolates, purifies and analyzes, RNA, DNA and protein including hybridization, gel electrophoresis autoradiography, ECL staining, performing PCR reactions, quantitative RT-PCR assay, DNA sequencing and assays for signal transducing enzymes.
• Purification and conjugation of immunoglobulins. Have basic knowledge of solution and buffer preparation in which ionic concentration and pH is critical. Have basic training in use and safety of radioisotopes.
• Get trained and learn how to perform DNA library preparation for single-cell sequencing using Tapestri® platform. Use the platform in routine basis to perform single-cell DNA sequencing. Coordinate with Core Facility to conduct next generation sequencing analysis.

Tissue Culture

• Maintains and establishes tissue culture cell lines which require good sterile techniques and the ability to observe and identify cell changes under the microscope, determine cell counts on the hemocytometer or coulter counter, performs colony counts or determine cell viability. Prepares sterile media and solutions.
• Monitors, and shelves tissue culture supplies to ensure efficient functioning. Prepare cells for cryogenic storage and reestablishment by the use of small vials, dry ice and liquid nitrogen.

Laboratory Equipment, Maintenance and Supplies

• Performs routine maintenance of ultra-low freezers, liquid nitrogen, C02 and water levels. Should be able to maintain equipment such as high-speed centrifuges, pH meters and microfuges.
• Manually operates and maintains the lab equipment. Visually reads equipment operation manuals and procedure manual whenever necessary. Must be able to lift and carry items up to 20-25 pounds and carry or move from one area of the lab to another by using a roller cart. Must be able to comply with all institutional safety policies including Universal Precautions and OSHA standards when handling toxic, hazardous, flammable, radioactive or potentially infectious or carcinogenic materials. This may require wearing common protective garments or equipment such as gloves, eye protection, face mask, gown or lab coat, etc. Additionally some procedures may require the use of a fume hood or biological safety cabinet.
• Assists with lab supply ordering, maintenance, and organization.

Scientific literature, Skill Building and Data Organization

• Ability to search and review scientific literature for the purpose of conducting and mastering experiments.
• Ability to engage and master novel experimental technologies.
• Entering data in concordance with methods requested
• Confer with superiors regarding results obtained.

EDUCATION

Required: Bachelor's degree with major course works in one of the basic sciences.

Preferred: Master's degree with major course works in one of the basic sciences.

EXPERIENCE

Required: Two years experience in scientific or experimental research work. No experience required with preferred degree.

Preferred: Experience with tissue culture and molecular techniques.

It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html