Principal Scientist, Cryo-EM Crystallographer

Boulder, Colorado
May 02 2021
Position Type
Full Time
Organization Type

We are seeking two structural biologists with strong expertise on X-ray crystallography and X-ray crystallography combined with Cryo-EM to join the Structural Biology group at Pfizer-Boulder Research and Development Unit. The successful candidate will apply a broad range of structural biology tools to determine high resolution ligand-bound protein cryo-EM and X-ray crystal structures to support our Structure-based drug discovery programs.

  • Grow diffraction-quality crystals of protein: ligand complexes for both novel and well-established targets by working with multiple crystallization formats to find optimal crystallization conditions for each target
  • Design and conduct protein crystallization trials, crystal optimization, and X-ray data collection using both in-house X-ray generators and remoted data collection on synchrotrons.
  • Solve and refine structures either by Molecular Replacement or de-novo
  • Optimize Cryo-EM sample vitrification conditions and collect high magnification data.
  • Coordinate and manage cryo-EM data collection on multiple protein targets.
  • Document experimental data and communicate research priority, progress, and results with project teams.
  • Contribute to written and oral presentations related to research findings.
  • Provides technical and scientific guidance to other members within the Structural Biology group


  • PhD in structural biology or a related field having 5+ years' postdoctoral experience with crystallography in industrial or academic research that lead a proven track record of achievement and publications in reputable, peer-reviewed journals
  • Extensive experience in protein X-ray crystallography from crystallization, optimization, data collection and processing, to structure solution.
  • Experience with software for model building and refinement such as Phenix, Autoproc, CCP4, Chimera, Rosetta, Coot.
  • Experience with crystallization robot
  • Experience with cryo-EM sample preparation protocols including negative staining and sample vitrification.
  • Experience with operating screening and high-resolution data-collection microscopes using programs such as EPU, SerialEM, and Leginon.
  • Expert in single particle cryo-EM image processing and 3D reconstruction using programs such as RELION, CryoSPARC, EMAN2.

  • 2+ year of postdoctoral cryo-EM experience capable of data-processing and cryo-EM structure solution, particularly with small size protein and transient membrane protein complexes (
  • Experience with Linux OS and GPU-accelerated computational environment.
  • Experience with in-house X-ray generator operation and maintenance.
  • Experience to use integrated biophysical and structural biology experiments to understand interactions between small molecules and macromolecular target.

  • Able to lift 50lb objects with dimensions 12 inches in height and 26 inches in width
  • Able to visualize microscopic objects under microscopes

  • Able to adhere to safe work practices and perform lab works with appropriate personal protection equipment such as but not limited to safety goggles, facial mask, gloves, lab coat/ protective gowning, and closed toe shoes
  • Able to work at flexible hours occasionally when performing time-sensitive experiments

  • Eligible for Employee Referral Bonus
  • Eligible for Relocation


The annual base salary for this position ranges from $90,500 to $150,900. In addition, this position offers an annual bonus with a target of 15% of the base salary and eligibility to participate in our share based long term incentive program. Benefits offered include a retirement savings plan, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage in accordance with the terms and conditions of the applicable plans.

Sunshine Act

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EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development


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