Associate Scientist, Structural Biology

Boulder, Colorado
April 29 2021
Position Type
Full Time
Organization Type
Why Patients Need You

Pfizer's purpose is to deliver breakthroughs that change patients' lives. Research and Development is at the heart of fulfilling Pfizer's purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best in class medicines to patients around the world.

What You Will Achieve

Pfizer-Boulder Research and Development is a fully integrated research unit in Boulder Colorado dedicated to the discovery and development of targeted small molecule drugs for patients afflicted with cancer. The Structural Biology group within Pfizer-Boulder R & D has a position for a junior level scientist with cell culture experience. This scientist will be responsible for maintaining and scaling up (≥5L) insect cell, mammalian cell, and sometimes E. coli cultures for recombinant protein expressions. A secondary role is generating, expanding, and assessing baculovirus stocks to enable large scale expressions. The individual may also be involved in molecular cloning and basic protein analysis and characterization. Knowledge in cell biology and experience in handling mammalian and insect cell cultures are essential for this role.

How You Will Achieve It
  • Passage suspended insect and mammalian cell cultures.
  • Generate and maintain baculovirus stocks for expressing recombinant proteins in insect cells.
  • Perform titrations and flow cytometry to determine viral titer.
  • Evaluate and optimize protein expression in insect cells, mammalian cells, and E. coli.
  • Multi-liter scale of protein expressions in insect cells and E. coli.
  • Document and maintain records on constructs, strains, and protein expressions.
  • Perform molecular cloning and DNA preparation for transfection.
  • Exercise and build basic team effectiveness skills {e.g., commitment, feedback, consensus management} within the immediate work group.
  • Assist in ordering supplies and stocking laboratory.
  • Present progress to colleagues.
  • Collect and analyze data to support troubleshooting and process improvement.
  • Stay current on all departmental and corporate training.


  • BS in Cell Biology, Molecular Biology, Biochemistry or related biological science
  • Experience routinely passaging cell cultures within an industrial or academic research institute setting (cell culture experience from a lab course does not qualify).
  • Proficient in Microsoft Office.
  • Able to work independently yet cooperatively; enthusiastic about learning new biology and techniques, and readily apply those learnings to immediate challenges.
  • Excellent communications and documentation skills.

  • 1 year of cell culture experience, insect or mammalian cells preferred.
  • Knowledgeable of flow cytometry and experience with designing multicolor panels.
  • Experience in insect or mammalian cell transfection and protein expression in fore mentioned hosts.
  • Experience in developing and troubleshooting assays.
  • Performed gel electrophoresis for DNA and protein analysis and immunoblotting.
  • Knowledge of molecular biology techniques such as cloning, PCR, and transformation.

  • Able to lift 50 lb objects with dimensions 12 inches in height and 26 inches in width.
  • M ust be able to wear PPE (lab coat, safety shoes/glasses/etc).

  • Must have flexible work schedule as some weekend work required

  • Last Date to Apply for Job: May 4, 2021
  • Eligible for Employee Referral Bonus

The annual base salary for this position ranges from $48,000 to $80,000 . In addition, this position offers an annual bonus with a target of 7.5 % of the base salary. Benefits offered include a retirement savings plan, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage in accordance with the terms and conditions of the applicable plans.

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development

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