The Hatten Lab seeks a postdoctoral researcher with strong stem cell and molecular biology background. This is an exciting opportunity for a candidate with a human stem cell background to gain expertise in developmental neurobiology, proteomics and transcriptomic methods, bioengineering and imaging of neural circuits. The candidate will work on a project to create a 3D, hPSC-based platform to model the human cerebellar circuit. To generate neuronal laminae of human cerebellar neurons, we will use to protocols in place in the Hatten Lab to generate cerebellar neurons from human embryonic stem cells (hPSCs) and state of the art bioengineering methods to develop a defined 3D culture system.
Using the mouse cerebellar cortex as a model for central nervous system development, Dr. Hatten has studied the molecular control of neurogenesis and fate specification during the early stages of brain development, and of glial-guided migrations in later developmental periods. Dr. Hatten pioneered the development of video imaging methods that provided the first evidence that the mode of CNS neuronal migration along glial fibers is conserved across brain regions, as well as a dynamic view of glial-guided neuronal migration and the key steps, cellular features, and molecular mechanisms involved. Her lab also co-established GENSAT with Dr. Nathaniel Heintz, that generated almost 1000 lines of transgenic mice to generate a gene expression atlas of the central nervous system, and also generate a large number of specific CRE lines, based on bacterial artificial chromosome methodology, and an invaluable resource to the scientific community. Recently, the lab has developed protocols to differentiate hPSCs and iPSCs into human cerebellar Purkinje cells and granule cells.
- Strong background in human stem cell biology is desirable, expertise in imaging and neurobiology preferred.
- Extensive experience with some of the following cell and molecular biology techniques is required: human stem cell culture, mammalian cell culture, PCR, RT-PCR, cloning, viral transduction, CRISPR-Cas9 gene editing, protein expression, immunoprecipitation, SDS-PAGE, and Western-blot.
- PhD degree in Stem Cell Biology, Neurobiology, Molecular biology or a related field of biology is required.
Please e-mail your CV and a cover letter to Prof. Mary E. Hatten (email@example.com) and arrange for reference letters to be sent directly to Prof. Hatten.
The Rockefeller University is an Equal Opportunity Employer with a policy that forbids discrimination in employment for protected characteristics. The Administration has an Affirmative Action Program to increase outreach to women, minorities, individuals with disabilities, and protected veterans.
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