Sr Research Assistant

Location
Houston, Texas
Salary
Competitive
Posted
April 22 2021
Ref
139379
Organization Type
Healthcare/Hospital
The University of Texas MD Anderson Cancer Center in Houston is one of the world's most respected centers focused on cancer patient care, research, education and prevention. It was named the nation's No. 1 hospital for cancer care in U.S. News & World Report's 2019 rankings.

***Ideal candidates will have experience working with mouse models***

1. Organizes and performs routine procedures involving molecular biology, tissue culture cell maintenance and storage and biochemistry of RNA, DNA and protein processing. Requires experience in tissue culture, DNA & RNA preparation and cloning, PCR, protein cell lysate preparation, protein synthesis and electrophoresis, MTT, western blot and clonogenic assays. Responsible in communicating experimental design and results.

2. Responsible for conducting daily animal work using mice tumor models including
• Injects tumor or normal cells into tongues, body flanks and tail veins.
• Ear tag and prepare animals (weight, shave) for tumor cell injection into tongues, body flanks and tail veins.
• Implants patient derived tumors (PDX) into the flank.
• Administer agents via IP, oral gavage and tail vein to study tumor growth inhibition with and without agent treatment.
• Performs immunohistochemistry on frozen and paraffin tissue sections.
• Breeds and maintains transgenic mice colonies and performs mice genotyping.
• In charge of login in-vivo data into frozen and paraffin tissue database.
• Orders mice from vet med via eSirius software
• Helps to submit for IHC requests to iLab.
• Handles correspondence between husbandry and vet staff regarding mice health concerns.
• Trains new personnel in mice experiments.

3. Performs and coordinates scientific experiments in a collaborative effort with other technicians, postdoctoral fellows, and staff members. Monitors workflow as it pertains to the need for supplies including ordering of all supplies, equipment maintenance and stock solutions.

4. Equipment monitoring on a daily basis verifying correct temperatures and maintenance of all freezers including liquid nitrogen tanks., refrigerators, incubators, etc. Performs preventive maintenance on laboratory equipment. Maintains all safety precautions and maintain lab safety records and assure that safety procedures are implemented by all lab personnel. Responsible for assigning Lab duties and general lab maintenance. Assumes responsibility for training lab personnel in routine safety practices.

5. Assemble samples, reagents, dry or wet ice, data and packaging materials; draft a cover letter, accurately and legibly fill out shipping labels. Keep accurate legible logs of outgoing and incoming packages. Ship all Hazardous and Infectious Materials in accordance with USA and Institutional policy.

6. Assists in Lab protocol management; MTAs, IBCs and Acufs

7. Computer skills: PowerPoint, Excel, Word

8. Other duties as assigned by PI and/or department laboratory manager

Education Required:
Bachelor's degree in one of the natural sciences or related field.

Education Preferred:
Master's degree in one of the natural sciences or related field.

Experience Required:
Three years of experience in scientific or experimental research work. With preferred degree, one year of required experience

Experience Preferred: working with mouse models

It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html

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