Pfizer

Protein Crystallization Specialist (non-PhD)

Employer
Pfizer
Location
Boulder, Colorado
Salary
Competitive
Posted
April 05 2021
Ref
4809085
Discipline
Other, Other
Position Type
Full Time
Organization Type
Pharma
ROLE SUMMARY

We are seeking a very experienced BS/MS degree level (non-PhD) protein crystallization specialist to join the Structural Biology group at Pfizer- Boulder Research and Development Unit. This individual will work closely with our protein crystallographer to express, purify, and crystalize a variety of challenging proteins for use in both structural and mechanistic studies.

ROLE RESPONSIBILITIES
  • Design and conduct protein crystallization trials, crystal optimization, and X-ray data collection.
  • Establish purification protocol and purify recombinant proteins from source cells.
  • Perform E. coli growth and recombinant protein expressions at liter-scale.
  • Document experimental data.
  • Communicate research priority, progress, and results with project teams.
  • Contribute to written and oral presentations related to research findings.
  • Provide technical and scientific guidance to junior research associates within the team.


BASIC QUALIFICATIONS
  • Bachelor of Science degree with 9+ years' or Master of Science degree with 7+ years' experience in protein crystallization and protein purification for crystallization.
  • An expert in protein crystallization resulted in diffracting crystals and high-resolution protein crystal structures.
  • Independent bench-scientist capable of purifying proteins at multi-mg scale; familiar with AKTA purifier system and standard analytical techniques associated with assessing protein purification process.
  • Experience in recombinant protein expressions in E. coli.
  • Strong organizational, problem-solving, and written/oral communication skills.


PREFERRED QUALIFICATIONS
  • Familiar with crystal handling and data collection at synchrotron facilities.
  • Experience with cryo-EM sample preparation protocols including negative staining and sample vitrification.


PHYSICAL/MENTAL REQUIREMENTS
  • Able to lift 50lb objects with dimensions 12 inches in height and 26 inches in width.
  • Able to visualize microscopic objects under microscopes.


NON-STANDARD WORK SCHEDULE, ENVIRONMENT REQUIREMENTS
  • Able to adhere to safe work practices and perform lab works with appropriate personal protection equipment such as but not limited to safety goggles, facial mask, gloves, lab coat/ protective gowning, and closed toe shoes.
  • Able to work at flexible hours occasionally when performing time-sensitive experiments.


OTHER JOB DETAILS
  • Eligible for Employee Referral Bonus
  • Eligible for Relocation


#LI-PFE

The annual base salary for this position ranges from $80,000 to $133,300. In addition, this position offers an annual bonus with a target of 12.5% of the base salary and eligibility to participate in our share based long term incentive program. Benefits offered include a retirement savings plan, paid vacation, holiday and personal days, paid caregiver/parental and medical leave, and health benefits to include medical, prescription drug, dental and vision coverage in accordance with the terms and conditions of the applicable plans.

Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development

#LI-PFE