Pfizer

Senior Associate Scientist, Cell Line Development

Employer
Pfizer
Location
Groton, Connecticut
Salary
Competitive
Posted
February 19 2021
Ref
4804855
Position Type
Full Time
Organization Type
Pharma
ROLE SUMMARY

We are seeking a highly motivated individual with strong expertise in molecular biology and cell line generation to join the Cellular, Genomic, and Protein Sciences (CGPS) group. This individual will join a collaborative team of scientists focused on the development and characterization of physiologically relevant cell models. This will include CRISPR/Cas9 gene editing, generation of clonal lines, and culture of primary cells and induced pluripotent stem cells (iPSCs). The candidate will also provide key molecular biology support to enable cell line development and characterization, and he/she will help drive continuous improvement in methods and technologies to ensure the highest quality and efficiencies in cell reagent provision.

ROLE RESPONSIBILITIES

The successful candidate will join a team of scientists within the CGPS group that is responsible for the development of cellular models to drive discovery programs across diverse therapeutic areas. He/she will provide molecular biology support for novel cell line generation and will execute standard cell culture processes including transfection, selection, sub-cloning, clonal expansion, and banking. As part of cell line characterization, the successful candidate will regularly apply techniques such as DNA isolation and sequencing, qRT-PCR, Western blotting, FACS, and immunostaining. He/she will also be involved in the production and support of more complex, disease relevant cellular models from human primary cells or iPSCs.

Successful Candidates will be expected to:
  • Demonstrate basic laboratory skills to propagate and maintain various continuous and primary cell lines including preparation of medium and mycoplasma testing to ensure cell line sterility.
  • Be adept at handling human primary cell models and enable the growing application of more physiology relevant models for cellular assays.
  • Develop stable cell lines by conducting transfections, selection, single cell cloning, screening, characterization, and clone expansion.
  • Provide molecular biology support for generating and characterizing, and screening cell clones including DNA/RNA extraction, plasmid preparation and cloning, qRT-PCR, FACS, and sequence analysis.
  • Oversee the operation, maintenance, and troubleshooting of key technologies and instruments for cell line development and characterization.
  • Help to identify, evaluate, and, where applicable, implement novel methods or technologies that would enhance workflow efficiencies and/or improve the quality of cell reagent deliverables.
  • Analyze and interpret data, prepare cogent data summaries, and communicate results to partners in the Research Units.
  • Maintain up-to-date electronic laboratory notebooks and records in accordance with Pfizer policy, and ensure proper archiving of banked cell vials.
  • Be highly motivated, organized and show significantly ability to work effectively and collaboratively in a team-oriented environment.


BASIC QUALIFICATIONS
  • BS or MS with 2-5 years relevant laboratory experience required.
  • Proficient in aseptic mammalian cell culture.
  • Experienced in the generation, characterization, and expansion of single-cell clones.
  • Proficient in standard methods for cell characterization including mRNA and protein analysis such as qRT-PCR, Western blotting, FACS, and immunostaining.
  • Hands-on molecular biology experience including nucleic acid/protein isolation, genomic DNA analysis and sequencing.
  • Understanding of CRISPR/Cas9 gene editing.


PREFERRED QUALIFICATIONS
  • Previous hands-on experience with CRISPR/Cas9 gene editing technology.
  • Previous hands-on experience with cell automation equipment including automated clone picking, clone consolidation, and kinetic imaging systems (Incucyte).
  • Previous experience with human primary cell models and/or iPSCs and iPSC-derived models.
  • Ability to effectively participate in cross functional collaboration and demonstrate excellent verbal and written communication skills.
  • Excellent work planning, organization and record keeping is essential.
  • Ability to multi-task and to balance bench and desk activities.


PHYSICAL/MENTAL REQUIREMENTS
  • Sitting, standing, lifting, bending, and walking.
  • Ability to perform practical laboratory bench work and work on a computer (while standing or sitting).
  • Ability to perform mathematical calculations and interpret data.


NON-STANDARD WORK SCHEDULE, TRAVEL OR ENVIRONMENT REQUIREMENTS
  • Minimal, if any travel related to this position.
  • Candidate may periodically be asked to travel to the Pfizer Cambridge, MA site to meet project team members.
  • Candidate must comply with laboratory safety practices.


Other Job Details:
  • Last Date to Apply for Job: April 1, 2021
  • Eligible for Relocation Package: YES
  • Eligible for Employee Referral Bonus: YES


Sunshine Act

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.

EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

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