Research Assistant II - Translational Molecular Pathology

SUMMARY

The primary purpose of the Research Assistant II position is to assist in various laboratory experiments including assisting in tissue harvest of mice, as well as assist the laboratory in performing various molecular and immunological analyses of human and mouse samples including cell culture, cell transfections, immunohistochemistry, immunofluorescence, flow cytometry, QPCR, and western blotting. The RAII will also assist in acquiring viable single- cells from human and murine tissues as well as generate single-cell expression and immune repertoire libraries. The RAII must be able to provide technical and coordination skills in carrying out various research projects, coordinates procedures, and performs technical laboratory duties in accordance with general and/or specific plans, with the main focus on molecular and cellular biology work.

JOB SPECIFIC COMPETENCIES

Laboratory Procedures Standard laboratory procedures including but not limited to: cell culture, cell transfections, bead-based Luminex assay, ELISA, western blotting, PCR/RTPCR, extraction of DNA, RNA, and protein from tissues and cells, preparation of viable single-cells from human and mouse tissues, generation of single-cell expression and immune repertoire libraries from single-cells and their preparation for sequencing.

Tissue Culture Basic tissue culture including work with retroviruses and lentiviruses.

Laboratory and Animal Maintenance Maintains Lab Functions to include ordering of supplies and ensures lab equipment is maintained in good operating order. Assist the lab in animal experiments if needed.

Collaboration Work as a member of a team and assist coworkers in the execution of their research projects. Must have excellent command of oral and written English skills.

Bachelor's degree with major course works in one of the basic sciences. Two years experience in scientific or experimental research work. No experience required with preferred degree. It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html