Scientist - High-throughput Protein Production

Cambridge, Massachusetts
November 26 2020
Position Type
Full Time
Organization Type
Pfizer's BioMedicine Design (BMD) High-Throughput Protein Production (HTP) group is seeking a highly motivated logical scientist with experience in protein purification, cell culture and molecular biology. The candidate will play a critical role in a core group responsible for high-throughput cloning, expression & purification of hundreds of antibodies on a microgram-to-milligram scale and be an advocate for utilizing various automation platforms. The candidate will be responsible for high throughput protein production, quantitation and, characterization as well as high-throughput cloning, interacting with project teams and updating of the laboratory database. The candidate will contribute to group-wide deliverables, work on several projects simultaneously, and present results and updates at group and project meetings.

  • High-throughput automated antibody purification, quantification and characterization using liquid handling automation.
  • Optimization of the automated processes throughout the HTP workflow.
  • HTP Mammalian cell culture, cloning and mammalian transfection using automated platforms.
  • Organization and storage of DNA and protein samples, including tracking using Genedata Biologics Software, as well as record keeping using electronic laboratory notebooks
  • Close interaction with protein engineering project teams to plan and coordinate protein production.
  • Regular review of literature to m aintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods.

  • Bachelors Degree with 6+ years relevant experience OR Master's Degree with 4+ years relevant experience.
  • Relevant experience includes; Molecular Biology, Biochemistry, Biology, Biological Engineering or a related discipline along with a basic understanding of antibodies and protein production.
  • Track record of strong problem solving and organizational skills, orientation to detail.
  • Ability to multi-task, prioritize responsibilities, work under strict deadlines, and remain flexible with changing needs.
  • Ability to work effectively to solve process development problems and implement improved procedures.
  • Lifting, sitting, standing, walking, bending, ability to perform mathematical calculations and ability to perform complex data analysis.
  • Strong written and oral communication skills.

  • 2 years of experience and demonstrated technical proficiency in protein purification using affinity and traditional chromatographic techniques preferred.
  • 1 year of experience with using laboratory automation, including writing and troubleshooting protocols preferred.
  • 2 years of experience and demonstrated technical proficiency in molecular biology: PCR, molecular cloning, construct design, sequence analysis preferred.
  • Industry experience in molecular biology/antibodies/therapeutic protein purification
  • Experience with high throughput sample tracking and laboratory logistics software
  • Prior experience in therapeutic protein discovery and biochemistry research
  • Experience of using logic for problem solving
  • Experience writing and understanding methods for laboratory automation technologies
  • Understanding of state-of-the-art gene synthesis

Other Job Details:
  • Eligible for Employee Referral Bonus
  • #LI-PFE

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EEO & Employment Eligibility

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.

Research and Development

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