Postdoctoral Associate in cryogenic electron microscopy (cryoEM)

Location
Jupiter, Florida
Salary
Salary commensurate with experience.
Posted
November 23 2020
Position Type
Full Time
Organization Type
Academia
Job Type
Postdoc

Postdoctoral Associate in cryogenic electron microscopy

Position Description:
Postdoctoral Research Associate positions are immediately available for several projects including structure-function studies of cytoskeletal proteins that control signaling downstream of adherens junctions and focal adhesions with implications in cancer and heart diseases. Fellows will join an exciting research environment in new state-of-the-art facilities of the Florida campus of The Scripps Research Institute (TSRI), which is renowned in the Structural Biology field. The laboratory is recognized as a world leader in the cytoskeleton field.

The position provides an outstanding training program in protein biochemistry and structure, and also in molecular and cellular biology, in a highly dynamic and team-oriented research program. Successful candidates will work in a cutting-edge research environment that tackles this important new arena in structural and cell biology. The laboratory has outstanding facilities for cell biology, molecular biology, protein expression, purification, and biochemistry, and cryogenic electron microscopy and protein crystallography.

The laboratory collaborates with several other laboratories on campus including exciting collaborative and currently active projects with Drs Pat Griffin, Joe Kissil, Kirill Martenmyanov, Kendall Nettles, Mark Sundrud, and Susana Valente. Thus, there are several exciting opportunities for a variety of very interesting projects.

The Scripps Research Institute provides an incredibly rich scientific environment. Notably, TSRI faculty include an outstanding cadre of senior colleagues, including two Nobel Laureates and twenty Members of The National Academy of Sciences, including several in crystallography and biophysics. In 2008, The Scripps Research Institute moved into three new, state-of-the-art research buildings.

The Scripps Research Institute has a large cast of outstanding Cores that facilitate basic research and drug development. The unique infrastructure at The Scripps Research Institute includes the application of unique chemical, genomic, proteomic, informatics, and high-throughput screening and engineering tools for the discovery of novel biomolecules.

The Scripps Research Institute is a member of the South East Regional Collaborative Access Team (SER-CAT) that constructed two beam lines at the Advanced Photon Source (APS) at Argonne National Laboratory. The insertion device and bending magnet beam lines are available for all the synchrotron-related data collection needs for the post-doctoral fellows.

The laboratory recently installed the first 300 kV cryo Atomic Resolution Microscope (cryoARM300) in the Americas and has plenty of microscope time dedicated to the fellow. This state-of-the-art transmission electron microscope is a 300 KV high resolution microscope capable of carrying out cryo-electron microscopy studies by providing atomic resolution for structural studies involving single particle analyses. The transmission electron microscope is equipped with new and improved Gatan K3 direct electron detector camera that has better DQE, larger field of view (24 Megapixels – 5,760 x 4,092) and faster (3.75 times that of K2 camera). The direct detection devices are exquisitely sensitive, produce high signal to noise images, and are ideal for high-resolution studies.

The Scripps Research Institute provides a HighFlux X-ray diffraction system, specifically a Rigaku MicroMax-007 HF, a second-generation true microfocus rotating anode generator. Scripps also provides an Osmic VariMax optics system and the Proteros Free Mounting System to control the humidity and to optimize diffraction characteristics, including resolution, mosaicity, and anisotropy, as well as reduced X-ray background. Finally, the X-ray laboratory houses the mar345 Image Plate detector.

Palm Beach County is one of the most sought-after communities in the country and offers residents an incomparable lifestyle in a family-friendly community with public playgrounds and parks and access to world-class shopping and unmatched outdoor recreation. It is one of the safest communities in the United States with virtually no crime. The Atlantic Ocean with its 45 miles of spectacular beaches and signature Coconut Palms provides an exclusive getaway in a warm, sunny climate throughout most of the year. There are a number of exquisite art galleries as well as arts and crafts festivals. This golfer’s paradise is also home of the Professional Golfers Association of America (PGA).

Basic Qualifications:
Applicants with a recently awarded Ph.D. degree and experience in cryogenic electron microscopy (cryoEM) are probably a better fit for this position.
Please send CV, brief summary of research experience and interests, and names, addresses, e-mail, and telephone numbers of 3 references to:

Dr. T. Izard  
Professor
Cell Adhesion Laboratory
Department of Integrative Structural and Computational Biology 
The Scripps Research Institute
Jupiter, FL 33458
cmorrow@scripps.edu