Laboratory Manager - Reck-Peterson Lab
Howard Hughes Medical Institute (HHMI) is an independent, ever-evolving philanthropy that supports basic biomedical scientists and educators with the potential for transformative impact. We make long-term investments in people, not just projects, because we believe in the power of individuals to make breakthroughs over time. HHMI scientists have radically advanced the understanding of cells, the brain, the immune system, the development of organs, and how to treat many diseases. Founded in 1953 by aviator and industrialist Howard R. Hughes, HHMI is headquartered in Chevy Chase, Maryland, and employs more than 2,500 people across the U.S.
At HHMI, you are not just an employee – you are a part of a creative and talented team with colleagues whose expertise ranges from biomedical research to investment management, from information technology to law. We encourage collaborative and results-driven working styles and offer an adaptable environment where employees can do their best work. As HHMI scientists continue to push boundaries in laboratories and classrooms, you can be sure that your contributions while working at HHMI are making a difference.
We are currently looking for a talented and enthusiastic Laboratory Manager to join the laboratory of Dr. Samara Reck-Peterson at the University of California, San Diego (UCSD) in San Diego, California.
Dr. Reck-Peterson’s laboratory studies the mechanisms of microtubule-based transport within cells. The microtubule cytoskeleton and associated transport machinery is responsible for organizing intracellular contents spatially and temporally. The Reck-Peterson lab strives to understand how this system works at molecular, cellular, and organismal scales, and to determine why defects in transport cause neurological diseases, including Parkinson's disease. They use a combination of approaches including protein biochemistry and in vitro reconstitution, single-molecule biophysics, proteomics, fungal genetics, mammalian cell culture including of ES cells, gene-editing of mammalian cells, and state-of-the-art live-cell imaging. Through collaborations, they have access to cryo-electron microscopy and cryo-electron tomography to pursue structural studies of the transport machinery. More information about the Reck-Peterson lab can be found here: http://reck-peterson.ucsd.edu/
Essential Duties and Responsibilities
- Perform research related to the molecular basis of intracellular transport and the role of LRRK2 in Parkinson’s disease.
- Use molecular biology techniques to clone new constructs.
- Use Western blots to evaluate protein expression and protein interactions.
- Fix and stain samples for immuno-fluorescence microscopy.
- Generate gene-edited cell lines using CRISPR/Cas9.
- Maintain multiple tissues culture cell lines, including ES and iPS cell lines.
- Collaborate with graduate students and postdoctoral fellows in the lab to pursue these research efforts.
- Evaluate new techniques and technologies.
- Manage day-to-day activities within the research laboratory, including such activities as ensuring operation of essential equipment and making sure adequate supplies are available for routine experimental procedures.
- Act as liaison between the lab, HHMI, and host institution administrative offices on issues such as budget management, fixed asset inventories, use of host accounts, and so forth.
- Act as liaison between the lab and host institution safety/regulatory offices to ensure compliance with all applicable regulations.
- Develop laboratory guidelines and maintain procedural manuals.
- Train incoming lab team members on proper lab conduct, technical areas, and complex processes as needed.
- Plan for and provide working supervision to Research Technician(s), other lab team members, and undergraduate students as needed.
• A bachelor’s degree in biological, biochemical, or biophysical sciences is required.
• Advanced degree would be a plus.
• A minimum of 3+ years of lab experience including at least one year of supervisory experience
• Experience in molecular biology and cloning is required.
• Experience in handling proteins, e.g. protein purification and Western blot analysis is
• Experience with mammalian cell culture is required.
Knowledge, Skills, and Abilities:
• Strong operational knowledge of advanced lab equipment, including centrifuges, microscopes,
and plate readers.
• Strong oral and written communication skills.
• Strong attention to detail with a high degree of accuracy in work.
• Ability to supervise and train others.
• Ability to initiate and compose communications for various purposes in an appropriate style.
• Ability to develop instructional material for routine training purposes.
• Ability to review goals, analyze processes, and develop techniques and systems required to
meet those goals.
• Ability to explore new areas and seek opportunities to apply new knowledge to work.
HHMI is an Equal Opportunity Employer.