A postdoctoral investigator position is available in the laboratory of Dr. Esther Guzmán in the Marine Biomedical and Biotechnology Research Program at Harbor Branch Oceanographic Institute at Florida Atlantic University in Fort Pierce, FL. This is a 1-year grant-funded position with the possibility of renewal for a second year based upon performance. The research that will be conducted will fulfill the aims in a grant supported by the Florida Department of Health to identify marine natural products that induce cytotoxicity in triple negative breast cancer cells grown as spheroids.
With over 30,000 students from all 50 states and over 180 countries, 21 intercollegiate teams, 180 plus degree programs, and an administration that thrives on discovery and excellence, FAU is pushing the boundaries of higher education. Florida Atlantic's faculty and staff are trailblazers and innovators with a passion for finding creative solutions while advancing science, art, and technology in all disciplines. Our work environment is a hub of cultures from across the nation and the world, making FAU a place where collaboration flourishes.
Summary of Responsibilities Include:
- Isolating protein for functional proteomic analysis for selected compounds and analyzing the data provided by the Functional Proteomics Reverse Phase Protein Array Core Lab performing the analysis.
- Preparing samples to conduct L1000 gene expression analysis and analyzing resulting data provided by the company that performs this analysis.
- Using databases such as LINCS and Connectivity Map from the Broad Institute and STRING from EMBL to analyze the proteomic and genomic data.
- Testing selected compounds on heterospheroids of triple negative breast cancer cells with fibroblasts or macrophages to address tumor-stroma interactions in a high content imager.
- Conducting 3D tumor spheroid invasion assays to determine if selected compounds affect metastatic potential.
- Validating proteomic/genomic results using siRNA, functional assays or flow cytometry and/or western blotting.
- Maintain a high level of productivity in the laboratory setting.
- Proactively seek out new information in the literature and incorporate this information into the individual project as well as the overall program.
- Prepare manuscripts on research findings for submission to high impact peer-reviewed journals and present work at scientific conferences.
- Demonstrate a high degree of responsibility in maintaining scientific standards and safe laboratory practices for self and reporting staff if any.
- Supervise one or more summer interns.
- Maintain accurate and current records of research and/or project related activities.
Ph.D. degree from accredited institution in biomedical sciences, cancer biology, immunology, cell biology or related discipline by the time of application required.
Strong laboratory and analytical skills.
A record of peer-reviewed publications, and proficiency in verbal and written scientific presentations.
Advanced knowledge of techniques such as sterile cell culture, microscopy, flow cytometry, cytotoxicity testing, protein and nucleic acid isolation, antibody labeling, ELISAs, techniques to measure angiogenesis and metastasis in vitro such as cell migration, cell scratch assays and endothelial cell tube formation assays, and molecular biology techniques routinely used in cell biology such as siRNA, western blotting and electrophoresis.
Proficiency in the use of high content imaging and analysis of data generated. Experience with cell culture in 3D-spheroids highly desired.
Advanced knowledge of bioinformatics and algorithms for analysis of high content imaging data and for analysis of proteomic and genomic differential analysis will be strongly preferred.
Key Leadership Competencies:
Strong interpersonal and communication skills with demonstrated ability to work within a team environment.
Builds strong relationship with peers and partners outside of team to enable higher performance.
Learns fast, grasps essential information quickly, and can change course quickly where indicated.
Raises the bar and is never satisfied with the status quo.
Creates a learning environment, open to suggestions and experimentation for improvement.
All applicants must apply electronically to the currently posted position (Postdoctoral Investigator in Cancer Cell Biology) on the Office of Human Resources' job website (https://fau.edu/jobs) by completing the required online employment application and submitting the related documents. When completing the online application, please upload the following: a cover letter, curriculum vitae, a two page statement of research, a list of at least three professional references including their contact information, and copies of official transcripts scanned into an electronic format.
Confidential letters of recommendation letters to be sent to Julie Pettingill, firstname.lastname@example.org.
A background check will be required for the candidate selected for this position. This position is subject to funding.
For more information and to apply, visit www.fau.edu/jobs and go to Apply Now (REQ08381).
Florida Atlantic University is an equal opportunity/affirmative action/equal access institution and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability status, protected veterans status or other protected status. Individuals with disabilities requiring accommodation, please call 561-297-3057. 711.
FAU is committed to the principles of engaged teaching, research and service. All persons aspiring to achieve excellence in the practice of these principles are encouraged to apply.
This position is funded with recurring grant funds with an expiration date on March 23, 2022. Renewal of funding is anticipated; however, not guaranteed.