Research Technician II-III, Vaccine Immune Response
Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
This research opportunity is with the McGuire Lab within the Vaccine & Infectious Disease Division. The technician will work under the direction of Dr. McGuire and use discretion and judgment in performance of duties in support of efforts to evaluate the immune responses to subunit vaccines against viral pathogens in multiple small animal models. In particular, the candidate will clone viral sequences into vectors, and express and purify recombinant proteins and perform immunizations. They will also be responsible for assisting in analyzing the immune response to these antigens including serological and neutralization analyses, as well as antigen-specific B cell sorting using FACS. The candidate will interpret and report on experimental results and assist with data presentation for conferences and manuscripts. The candidate will also work independently and train new recruits in the lab.
- Maintenance of cell lines
- Maintenance of murine colony
- Operate and carry out proper maintenance and QC of laboratory equipment
- Maintain reagents, stocks, and cultures necessary to perform work
- Order and maintain laboratory supplies
- Cloning, sequencing and validation of various expression plasmids
- Co-ordinate immunization protocols with contract research organizations and with lab members
- Production of recombinant protein from large volumes of culture (>2L) by transient transfection under sterile conditions
- Purification of various viral proteins using various chromatographic methods (IMAC and Lectin affinity, MMC, SEC)
- Coordinate sample shipping/receiving with collaborating laboratories
- Perform ex vivo infectivity assays on cell lines and primary tissues, and train laboratory staff to be proficient in them
- Perform reporter assays to measure viral infection, and train laboratory staff to be proficient in them
- Be available to process murine serum and tissue specimens as needed Prepare and cryopreserve PBMC
- Perform and develop flow cytometric assays (e.g. both phenotyping and functional assays)
- Perform and develop ELISAs and related assays to measure vaccine-specific antibody responses
- Independently collect and analyze data
- Help with production of manuscripts for new assays and studies by taking a significant role in writing, data analysis, making figures and interpretation of data Identify methodological problems in research protocol and implement modifications to optimize experimental results
- Prepare and present written or oral progress reports concerning studies including assembly, organization, and interpretation of data
- Conduct literature reviews to assist in determining most suitable methods to be used in research Other duties as assigned
- Bachelor’s degree and 2+ years’ relevant experience working in a research laboratory. Master’s Degree acceptable in lieu of experience requirement.
- Murine colony management, specific hands-on experience with murine models including breeding, genotyping, tracking, immunizations, bleeds and tissue harvests
- Mammalian cell culture, Tissue culture
- Must be willing to work with biohazardous/infectious agents (e.g., blood-borne pathogens, including HIV-1), including human tissues under carefully controlled conditions.
- Must be flexible and willing to work some evenings and weekends.
- Motivation and adaptability are essential, as is a keen interest in areas of vaccine development and prevention.
- Independent research capabilities and strong organizational and communication skills are necessary.
- Bachelor’s degree in a biological science and 6+ years of previous experience working in a laboratory and/or a master’s degree with 4+ years’ experience.
- Cloning including Gibson assembly and ligation-based
- Flow cytometry and sorting (FACS)
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