Under the direction of the Principal Investigator (PI), the Research Associate/Instructor will perform basic science research in the Department of Pharmacology in the College of Medicine. This position involves participation in a variety of research activities on the role of ion channel-coding cDNAs and the resulting expressed proteins (e.g., BK channel different subunits, RyR1-3, TRPV1), and their modification by physiologically relevant lipids and pharmacological agents.
DUTIES AND RESPONSIBILITIES:
- Conduct PCR, real-time PCR, construct vectors, DNA subcloning and in vitro transcription of recombinant DNA.
- Design point mutants and chimeric channel subunits to determine amino acid participation in ligand recognition.
- Conduct cell transfection, Western-blotting, immunohistochemistry, and other methods to identify protein expression.
- Conduct cDNA nuclear and cRNA cytoplasmic injections in frog oocytes.
- Conduct sucrose gradients, protein enrichment, and ion channel reconstitution into lipid bilayers.
- If needed, conduct simple experiments using robotic or lipid bilayer electrophysiology.
- Conduct protein-protein interaction studies using affinity chromatography or FRET.
- Supervise and keep maintenance of all devices and materials related to recombinant DNA work and other techniques mentioned above.
- Maintain detailed log book of experiments.
- Supervise work of students, fellows, and junior employees in the laboratory.
MINIMUM QUALIFICATIONS: PhD. in Biochemistry, Molecular Biology or related sciences; three (3) years experience in a biomedical research laboratory. The candidate must have documented proficiency (publications) with recombinant DNA, in particular with mutagenesis and expression of long DNA sequences.
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