TECHNICAL ASSISTANT (m/f) in Cell Line Engineering and Sample Quality Control
The Superti-Furga laboratory at CeMM in Vienna is contributing to the scientific fundaments of precision medicine by studying drug action at the systems-level. In recent years, the laboratory has focused on understanding how membrane transporters govern access to nutrients, ions, xenobiotcis and drugs for cells and organisms. Regulation of the function of transporters is integrated with metabolic processes, brokering internal demand and external offer. Specificity, regulation and functional integration of the roughly 1,500 human genes encoding various types of transporters is only poorly understood. The Superti-Furga laboratory is particularly interested in the > 400 members of the Solute Carrier Proteins super group (SLCs), as they contain some highly prominent drug targets and drug transporters, are involved in numerous diseases, but are dramatically understudied (César-Razquin et al. Cell 2015). According to the mission of the laboratory, SLCs can be considered the managers of the interface between chemistry and biology, and between organisms and their environment. Consequently, studying their regulation is of fundamental importance for biology and medicine.
As part of the Research Empowerment on Solute Carriers (RESOLUTE) consortium (www.re-solute.eu), an IMI-supported partnership of several leading academic laboratories and pharmaceutical companies that aims at “unlocking” the SLC target class, we are currently searching for a highly motivated research technician who wants to contribute to cutting-edge biomedical research with a focus on membrane transporters and their role in human physiology and pharmacology.
Requirements and desired qualifications
- or Master degree in life sciences
- wet-lab and/or quality control experience
- Experience in the culture of eukaryotic cell lines
- general knowledge of molecular biology techniques (PCR genotyping, cloning, immunoblotting)
- in MS Office
- accuracy and reliability
- good written and oral communication skills in English
- competence and the ability to work in a team
- Previous experience in generation and implementation of standard operating procedures is a plus
- Experience with cell line engineering (CRISPR/Cas9) is a plus
Your main mission will be to engineer a battery of cell lines using cutting-edge genome engineering technologies, control the quality of these cell lines and to manage and distribute these reagents to partner institutes. Previous experience with CRISPR/Cas9 and Jump-In technologies is an advantage. Very good command of English (in writing and speaking) is required. Your main tasks and responsibilities include:
- culture (maintenance, manipulation via genome engineering, cellular viability assays)
- molecular biology techniques (PCR, genotyping, immunoblotting, cloning)
- analysis using basic bioinformatics tools (blast, reading of trace files, TIDE)
- development as team effort with postdoctoral fellows
- of new lab members
- record keeping and report writing
- at team meetings and, occasionally, externally
- control of biological samples (cell lines, proteins, serum, DNA)
- Management and distribution of a large and complex collection of biological samples
Why you want to join our team
- is a great opportunity for junior researchers or experienced technical assistants who want to join a unique project in an international setting.
- appreciate an inspiring and dynamic working atmosphere, the possibility to join our cultural, social and sports activities and you want to be part of a creative, motivated team.
- are happy to provide help and assistance to all our employees, their spouses and family members in terms of visa and relocation procedures, dual-career and childcare services, and others.
- offer a fair employee benefits package including health insurance, company health care, competitive holiday allowance, daily bonus for the in-house cafeteria and an annual gross salary (according to the FWF personnel costs scheme https://www.fwf.ac.at/en/research‐funding/personnel‐costs/, depending on qualifications and experience).
- position is planned for the initial project duration of 2 years, that could be renewed
CeMM is the novel, international, independent and interdisciplinary Research Center for Molecular Medicine of the Austrian Academy of Sciences. Access to genomics, proteomics, immunology, cancer biology, vascular biology and signalling expertise, a formidable SAB as well as close collaborations with the clinical departments of the Medical University of Vienna are part of the opportunity. CeMM is proud to be ranked as the best European place to work in Academia, according to a survey by The Scientist that was published on August 1, 2012. Vienna is a particularly enjoyable and international capital, with the worldwide #1 quality of life (MERCER), world-best classical music, great food and outdoors and the best coffee/wine/beer combination of any city in the world. The official language at CeMM is English. CeMM aims to promote equal opportunity for all, with the right mix of talent, competences and potential. We welcome applications from candidates with diverse backgrounds.
Please apply online with a cover letter, your detailed curriculum vitae and contact details of 2-3 referees. Please upload your full application here: https://cemm.jobbase.io/job/6op3vwh9
Ideally, you should please submit one PDF file containing all of your application documents. Initial application deadline is October 26, 2018 but the call will remain open until the position is filled. Candidates will be contacted by us on a rolling basis.