Postdoctoral Research Fellow - Immuno/Oncology of Breast Cancer
We seek motivated postdoctoral fellows interested in pursuing cancer immunology research in the lab of Dr. Geeta Upadhyay at Department of Pathology, Uniformed Services University of Health Sciences (USUHS), Bethesda, MD.
About USUHS: The mission of the USUHS is to educate, train, and comprehensively prepare uniformed services health professionals, scientists, and leaders to support the Military and Public Health Systems, the National Security and National Defense Strategies of the United States, and the readiness of our Uniformed Services. USUHS is integral part of F. Edward Hébert School of Medicine. Within campus, we collaborated with Armed Forces Radiobiology Research Institute and with Murtha Cancer Center, Walter Reed, capital Breast care project. USUHS is located in close proximity to National Institute of health, providing opportunities for cross campus collaboration. More info: https://www.usuhs.edu/
Core facilities at USUHS: https://www.usuhs.edu/research/core-facilities
About Geeta Upadhyay: Dr. Upadhyay recently joined USUHS faculty as tenure track faculty. Dr. Upadhyay received her training at Harvard, NCI, UTHSCSA, Georgetown University. Her research is focused on biomarker discover, drug development and drug resistance in breast cancer.
PROJECT DESCRIPTION: The postdoctoral candidate will have the opportunity to conduct some of the research described in our newly funded R01 grant title “Role of Ly6K in TGF-beta and immune escape pathways of triple negative breast cancer”. Ly6K, a GPI anchored protein, is expressed in cancer tissue and is required for in vivo tumor progression. We have shown that Ly6K activates TGF-beta signaling, epithelial-to-mesenchymal transition and PD-L1 expression. We found that knockdown or pharmacological inhibition of Ly6K by small molecules binders, can significantly inhibit multiple distinct aspects of the biological activity of Ly6K. This proposal will be focused on determining the molecular basis of Ly6K action and Ly6K targeted therapeutics.
Flow cytometry: Sample prep, execution on the machine, data analysis, sorting and multicolor panel
Cell culture: Primary and cell lines, T cell expansion.
In vivo studies: Mouse handling, i.p. injections, tumor injections and
measurements, immunotherapy assays, tissue collection and processing, mouse genetics.
In vitro and ex vivo assays: cell signaling, live cell imaging, westerns, qRT-PCR, luminex.
- Upadhyay G, Yin Y, Yuan H, Li X, Derynck R, Glazer RI. Stem cell antigen-1 enhances tumorigenicity by disruption of growth differentiation factor-10 (GDF10)-dependent TGF-beta signaling. Proc. Natl. Acad. Sci. U.S.A., 108, 7820–7825 (2011). PMID: 21518866.
- AlHossiny M, Luo L, Frazier WR, Steiner N, Gusev Y, Kallakury B, Glasgow E, Creswell K, Madhavan S, Kumar R, Upadhyay G. Lymphocyte antigens 6 complex K/E-transforming growth factor-beta axis promotes breast cancer tumorigenesis. Cancer Res., 76, 3376–3386 (2016). PMID: 27197181.
- Luo L, McGarvey P, Madhavan S, Kumar R, Gusev Y, Upadhyay G. Lymphocyte antigens 6 complex gene family members drive tumorigenesis. Oncotarget, 7, 11165–11193 (2016). PMID: 26862846
Click here to apply for the postdoctoral position at USUHS website.