Research Specialist II - Surgery: Center for Cellular Therapy

Charleston, South Carolina (US)
Band 5: $32,838.00 - $46,799.00 - $60,760.00 (min - mid - max)
June 14 2018
Position Type
Full Time
Organization Type

Research Specialist II - Surgery: Center for Cellular Therapy


Job Responsibilities

30% - Immunological testing of Human Blood and Tissue Specimens. This portion of the position will require performance of complex microbiological testing of blood specimens from clinical patients. This will include isolation of the buffy coat from whole blood using a density gradient separation and staining for Flow Cytometry. The isolated buffy coat is used to set up and run multiple types of immunological tests such as Tetrameric Peptide Staining, Cytotoxic T Lymphocyte Assay, Cytokine Bead Assay, ELISA and EliSPOT. The extracellular and intracellular staining of samples with multiple cell markers for Flow Cytometry will be for data collection and analysis.

30% - Processing of human blood and tissue specimens in a classified cleanroom/controlled environment. The employee will be involved in handling viral vectors; preparing vaccines; aseptic processing and large-scale culture of PBMCs, unstimulated mononuclear derived cells, stimulated peripheral blood-derived mononuclear cells, CD34+ selected cells, T-cells and/or other specific cells collected during apheresis. Cell isolation machines such as the COBE 2991 Cell Processor and the CliniMACS Cell Separator will perform the separation of the target cells. The target cells will be processed, cultured, packaged and/or cryopreserved.

30% - The employee will be involved in the Autologous Islet Cell Isolation process, in which Islet cells are isolated from a patient’s pancreas by enzymatic digestion and packaged for infusion.

5% - Other duties as assigned.

5% - Other general Lab Duties will include routine cleaning of the controlled environment using specified chemicals some of which require the use of a full-face respirator, managing inventory of supplies, ordering of supplies, instrumentation disinfection and sterilization, data collection, training and development of protocols, etc.