Postdoctoral position in protein engineering at the University of Washington
The laboratory of Dr. Liangcai Gu in the Department of Biochemistry and the Institute for Protein Design (IPD) at the University of Washington School of Medicine invites applications for a postdoctoral position in developing a high-throughput T-cell receptor (TCR)-ligand interaction quantification platform for the design of therapeutic TCRs. This position is available immediately.
TCRs are an emerging class of cancer therapeutics by serving as the key components in cell therapies as well as soluble biologics. TCRs recognize antigen-derived peptides presented on major histocompatibility complex (pMHC) molecules on antigen-presenting cell surfaces and thus are broadly applicable to extracellular and intracellular tumor antigen targets. Despite their importance, the design of TCRs with desired affinity and specificity for any given pMHC target to control T cell killing ability remains an unsolved problem. We are applying protein barcoding and in situ sequencing technologies, for example, a SMI-seq technology (Nature, 2014, 515:554–557; https://youtu.be/xaRKjTZY79U), to quantify interactions between TCR and pMHC libraries. We are developing the ‘library-by-library’ screening of single-chain TCR and pMHC class I libraries to study mechanisms governing TCR binding affinity and specificity. We will integrate the large experimental data sets into computational structure modeling to guide TCR design.
Applicants should have a Ph.D. degree (or equivalent) in related fields including, but not limited to biochemistry, immunology, biophysics, and bioengineering. Desirable backgrounds/skills include: i) protein display techniques such as phage and yeast display; ii) TCR functional study using tissue culture and mouse models, and iii) protein structure determination.
Applicants should have a strong publication record, good spoken and written communication skills. Interested applicants should send i) a cover letter briefly describing your previous experience and future research interests/plans, ii) a curriculum vitae, and iii) contact information of three references to Dr. Liangcai Gu (firstname.lastname@example.org).
Equal Employment Opportunity Statement
The University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information.