CBG Associate

February 22 2018
Life Sciences
Position Type
Full Time
Organization Type
About City of Hope

City of Hope, an innovative biomedical research, treatment and educational institution with over 5,000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy.

Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research. City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation's 20 leading cancer centers that develops and institutes standards of care for cancer treatment.

Requisition ID: 13002

Job Title: CBG Associate

Position Summary and Key Responsibilities:

CBG Associate (Center for Biomedicine and Genetics Associate) sought by Beckman Research Institute of the City of Hope in Duarte, CA.


  • Participate in the development of translational research in the field of cancer immunotherapies.
  • Manufacture clinical grade biological and cellular products including viral vectors, plasmid DNAs, recombinant proteins, and human stem cell-derived cellular products that will be used to support early phase clinical studies.
  • Maintain and passage cell cultures in addition to performing large-scale cell expansion in bioreactors or equivalent vessels.
  • Perform transfection procedures to produce viral vectors and differentiation procedures to produce the final products from human cells.
  • Perform analytical assays such as ELISA, flow cytometry, and PCR-based assays to support process development efforts.
  • Manufacture viruses such as lentiviral vectors, modified vaccinia Ankara (MVA) and adenovirus in a cGMP environment that will be used for clinical trials and translational research.
  • Apply knowledge of cGMP guidelines and good documentation process.
  • Manufacture activities including aseptic manipulation of multiple immortalized cell cultures including 293T cells, perform transfection procedures and virus infection of cells and concentration of the viral vectors using ultrafiltration and centrifugation techniques.
  • Determine viral infectious titer using flow cytometry analysis (FACs).
  • Perform process development to explore new technology for manufacturing lentiviral vectors.
  • Author, revise, and review documentation such as Standard Operating Procedures (SOPs) and Batch Records for manufacturing activities under time limitations.
  • Maintain detailed records of each production to comply with the regulatory requirements of cGMP and SOPs.
  • Lead multiple projects related to large scale lentivirus and adenovirus productions.

Minimum Education and Skills Required for Consideration:

  • Master's degree or equivalent in Biotechnology, Biological Science or a related field, plus Two (2) years of experience as a Research Associate, Research Technician, or related occupation within translational research field, including working on lentiviral vector productions using transient transfection procedures and titration of the viral vector to determine the infectious titer; maintaining and expanding multiple immortalized cell cultures including 293T cells, and preparing buffer solutions
  • Experience with transduction of cells with viral vectors and fluorescent microscopy
  • Creating, revising, and reviewing Standard Operating Procedures (SOPs) for virus productions and manufacturing procedures
  • Preparing manuscript and poster for scientific presentations
  • Participating in multiple projects and process developments, and leading a project from initiation to completion.

City of Hope is committed to creating a diverse environment and is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, or status as a qualified individual with disability.

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