Can there be only one? Exploring heterogeneity in RNA splicing at single-cell resolution (MACUALA...

Earlham Institute
October 05 2017
Life Sciences, Biology
Position Type
Full Time
Organization Type

Alternative splicing is a key mechanism by which cells generate functional diversity from individual genes, however, it is not yet will understood if individual cells express a single or multiple alternatively spliced isoforms. In this project, we aim to unravel the complexity of alternative splicing in blood stem cells - to see how these cells regulate expression of individual isoforms as part of their functional heterogeneity,

The successful applicant will develop and apply cutting-edge techniques in single-cell genomics to explore this question. The Macaulay group has world-leading expertise in single-cell genomics and a strong background in blood cell formation and function, and secondary supervisor Wilfried Haerty has expertise in developing bioinformatics tools for the analysis of single-cell data. The student will be trained in both wet- and dry- lab techniques to take a fundamental biological question and develop and apply new tools to provide an unequivocal answer.

The student will gain a unique and influential skill set in cellular genomics, through training in haematopoietic stem cell biology and isolation by (FACS), single-cell genomics, NGS technology development and bioinformatics. The student will work in the exceptionally well equipped labs at EI, within a group that has an excellent track record in novel single-cell technology development and the PI has a strong network of collaborators in haematology (Cambridge, UCL, Karolinska) and genomics (Sanger Institute, KU Leuven) as well as key industrial partners.

The ideal applicant will enjoy solving complex technical problems, be curious about new technologies as well as fundamental questions in biology, and have a willingness to train in new techniques, as well as training others.

This project has been shortlisted for funding by the Norwich Biosciences Doctoral Training Partnership (NRPDTP). Shortlisted applicants will be interviewed as part of the studentship competition. Candidates will be interviewed on either the 9th, 10th or 11th January 2018.

The Norwich Biosciences Doctoral Training Partnership (NRPDTP) offers postgraduates the opportunity to undertake a 4 year research project whilst enhancing professional development and research skills through a comprehensive training programme. You will join a vibrant community of world-leading researchers. All NRPDTP students undertake a three month professional internship (PIPS) during their study. The internship offers exciting and invaluable work experience designed to enhance professional development. Full support and advice will be provided by our Professional Internship team. Students with, or expecting to attain, at least an upper second class honours degree, or equivalent, are invited to apply.

For further information and to apply, please visit our website:

Funding notes

Full Studentships cover a stipend (RCUK rate: £14,553pa - 2017/8), research costs and tuition fees at UK/EU rate, and are available to UK and EU students who meet the UK residency requirements.

Students from EU countries who do not meet the UK residency requirements may be eligible for a fees-only award. Students in receipt of a fees-only award will be eligible for a maintenance stipend awarded by the NRPDTP Bioscience Doctoral Scholarships, which when combined will equal a full studentship. To be eligible students must meet the EU residency requirements. Details on eligibility for funding on the BBSRC website:

This job comes from a partnership with Science Magazine and Euraxess

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