Exploring RNA structural conformation space in living cells (DING_J18DTP2)

Employer
John Innes Centre
Location
Other
Posted
October 04 2017
Discipline
Life Sciences, Biology
Position Type
Full Time
Organization Type
Academia

RNA plays crucial roles in living cells, ranging from metabolite sensing, transcription, RNA processing to translation. Its function is determined together by its sequence and structure. RNA structure encodes another layer of information besides the genetic code and it influences every step in the gene expression program. Despite its importance, studying of RNA structures has long been limited by the low-throughput nature of RNA structure probing. Recently several genome-wide in vivo RNA structure profiling methods have helped us to better understand the effects of sequence variation on RNA structure and their biological roles in living cells.


However, current RNA structure profiling methods represent a mixture, or an ensemble of different structural conformations. This limitation prevents us from accurately predicting in vivo RNA structures. In this proposal, I will dissect the mixture of different structural conformations by both SNP-structure evaluation and thermodynamic conformation shifting studies. This proposal aims to investigate, for the first time, genome-wide structural conformation alteration in living cells. Furthermore, this proposed work will also study the impact of RNA secondary structure on gene expression. This study will involve a broad range of knowledge across multiple disciplines including bioinformatics, systems biology, biophysics, computational biology and nucleic acid chemistry.


This project has been shortlisted for funding by the Norwich Biosciences Doctoral Training Partnership (NRPDTP). Shortlisted applicants will be interviewed as part of the studentship competition. Candidates will be interviewed on either the 9th, 10th or 11th January 2018.


The Norwich Biosciences Doctoral Training Partnership (NRPDTP) offers postgraduates the opportunity to undertake a 4 year research project whilst enhancing professional development and research skills through a comprehensive training programme. You will join a vibrant community of world-leading researchers. All NRPDTP students undertake a three month professional internship (PIPS) during their study. The internship offers exciting and invaluable work experience designed to enhance professional development. Full support and advice will be provided by our Professional Internship team. Students with, or expecting to attain, at least an upper second class honours degree, or equivalent, are invited to apply.


For further information and to apply, please visit our website: http://www.biodtp.norwichresearchpark.ac.uk


Funding notes


Full Studentships cover a stipend (RCUK rate: £14,553pa - 2017/8), research costs and tuition fees at UK/EU rate, and are available to UK and EU students who meet the UK residency requirements.


Students from EU countries who do not meet the UK residency requirements may be eligible for a fees-only award. Students in receipt of a fees-only award will be eligible for a maintenance stipend awarded by the NRPDTP Bioscience Doctoral Scholarships, which when combined will equal a full studentship. To be eligible students must meet the EU residency requirements. Details on eligibility for funding on the BBSRC website:http://www.bbsrc.ac.uk/web/FILES/Guidelines/studentship_eligibility.pdf


 



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