The role of microRNAs in the peripheral circadian rhythm in cartilage (CLARK_U18DTP)
During ageing, the failure of physiological mechanisms to maintain cartilage homeostasis leads to an increasing risk of the degenerative joint disease, osteoarthritis (OA), a leading cause of disability in the UK. There are no effective disease-modifying drugs to treat OA and with an ageing population, OA is an increasing public health and economic burden with 17 million patients predicted in the UK by 2030.
MicroRNAs, as a class, have been shown to be involved in skeletal development and joint health. Similarly, the peripheral circadian rhythm in cartilage has also been shown to be involved in tissue maintenance. The role of microRNAs in maintaining the circadian rhythm in cartilage has not been addressed. We recently discovered that microRNAs known to have a role in cartilage homeostasis, are regulated in a circadian manner. Moreover, they regulate genes involved in establishing the circadian rhythm. This project aims to explore this in more detail at the molecular level both in cultured cells and in the mouse.
Techniques include: microdissection of joint tissues; histology and imaging; RNA purification and gene expression analyses; subcloning and site-directed mutation; mammalian cell culture; transfection; qRT-PCR; western blot.
See Yang N, Meng QJ. J Biol Rhythms. 2016 31:415-27. doi: 10.1177/0748730416662748
This project has been shortlisted for funding by the Norwich Biosciences Doctoral Training Partnership (NRPDTP). Shortlisted applicants will be interviewed as part of the studentship competition. Candidates will be interviewed on either the 9th, 10th or 11th January 2018.
The Norwich Biosciences Doctoral Training Partnership (NRPDTP) offers postgraduates the opportunity to undertake a 4 year research project whilst enhancing professional development and research skills through a comprehensive training programme. You will join a vibrant community of world-leading researchers. All NRPDTP students undertake a three month professional internship (PIPS) during their study. The internship offers exciting and invaluable work experience designed to enhance professional development. Full support and advice will be provided by our Professional Internship team. Students with, or expecting to attain, at least an upper second class honours degree, or equivalent, are invited to apply.
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Full Studentships cover a stipend (RCUK rate: £14,553pa - 2017/8), research costs and tuition fees at UK/EU rate, and are available to UK and EU students who meet the UK residency requirements.
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