Study of the response mecchanisms of foodborne pathogens and spoilage microorganisms to natural a...

Employer
ALMA MATER STUDIORUM - UNIVERSITA' DI BOLOGNA - - DIPARTIMENTO DI SCIENZE E TECNOLOGIE AGRO-ALIMENTARI
Location
Italy
Posted
October 03 2017
Position Type
Full Time
Organization Type
Academia

The research will be the study of the stress response mechanisms of several pathogenic and spoilage microorganisms after the exposure to sub-lethal concentration of essential oils or their bioactive compounds like citral, carvacrol, (E)-2-hexenal and thyme essential oil. Stress response will be evaluated both in model and food systems like fruit juices, analcoholic beverages and vegetable ready-to-eat foods. In the first step of the research, for each microorganisms and natural antimicrobial considered, the minimum inhibitory and bactericidal concentration (MIC and MBC) will be determined. The microbial response mechanisms will be investigated by the application of different analytical techniques including mass spectroscopy approaches and GC\MS-SPME. Also Molecular methods will be used .RT-qPCR and two-dimensional electrophoresis on acrylamide gels will be applied. The flow cytometry will be used for the evaluation of the physiological state of the entire microbial population.



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