Endocannabinoid/Endovanilloid System (EC/EV) in Human Osteosarcoma
Osteosarcoma (OS) is the most common primary malignant bone tumor in children and is characterized by an aggressive clinical course. The cell of origin is still far from clear. It could be either an Osteoblast or the Mesenchymal Stromal Cell. We have chosen to study EC/EV system in children affected with OS because of the emerging anti-cancer properties of EC/EV system and its well-known role in pain modulation and bone metabolism.
We will collect human OS cell lines and human OS primary cells derived from bone biopsy of patients underwent surgery. We will evaluate the expression of: CB2, TRPV1 and target genes involved in apoptosis, cell survival, invasion and cell cycle progression before and after treatments with selective CB2 and TRPV1 agonist (JWH-133 and RTX). The expression of these receptors and target genes will be first evaluated on m-RNA level by q-PCR and RNA Sequencing and for the once where a significant difference in expression will be observed, also by Western Blot.
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