Genotoxicity assessment of titanium dioxide (TiO2) nanoparticles in human spermatozoa in vitro
Among the varieties of engineered nanoparticles (NPs), titanium dioxide (TiO2) nanoparticles are one of the most widely used in consumer products. As regards its massive presence in the environment, the attention of the study will be focused on the impact on male reproduction. It is known that n-TiO2 are able to cross the blood-testis barrier induced inflammation, cytotoxicity and gene expression changes, that lead to impairment of male reproductive system in mouse. The goal of this project is to investigate the genotoxic effects of TiO2-NPs on human sperm in vitro and the role of ROS as mediators of n-TiO2 genotoxicity by using different experimental approaches: Comet Assay, TUNEL test, DCF Assay, RAPD-PCR. The results of this study may provide useful information to evaluate the genotoxic potential of the nano-contaminant on human sperm, moreover, they could identify the possible pathways of activity of these contaminants on reproductive function in humans.
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