ILRS_06/2017: Doctoral Researcher (m/f) Nuclear identity and nuclear migration in Schizophyllum c...

Employer
Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute -
Location
Germany
Posted
September 26 2017
Discipline
Life Sciences, Biology
Position Type
Full Time
Organization Type
Academia

The Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute - in cooperation with the Friedrich Schiller University, the University Hospital Jena and the Max Planck Institute for Chemical Ecology are offering an international graduate training programme. The International Leibniz Research School (ILRS Jena) gives doctoral researchers the possibility to prepare for their PhD exam in an ambitious program providing excellent research conditions. We invite applications for a


Doctoral Researcher (m/f)


Nuclear identity and nuclear migration in Schizophyllum commune

Within the kingdom of fungi, the Dikarya (Ascomycetes and Basidiomycetes) show specifc development. One of the long-standing puzzles is the establishing of nuclear identify through the prolonged multi-nuclear life stage. Preceding the assortment of the two different nuclei for the formation of sexual spores during meiosis, a pronounced and fast nuclear migration process takes places. Here, as well, the nuclear identity must be kept and the migrating nucleus undergoes multiple mitotic events. Genetic evidence collected from the 1920s suggested that even in matings of dikaryons with a new monokaryon, the nuclear pair of a dikaryon can be newly composed by splitting the association previously found in the dikaryon and re-assorting two new nuclei for building a new dikaryon. Again, nuclear migration is necessary during this step.


The nuclear migration in basidioymcetes has been clocked with up to 4 cm/h, translating into 10 µm per sec, a movement that is at the upper limit of transport along microtubules. We have previously analyzed components involved in this process, establishing labelled nuclei (histone 2B fused to GFP), microtubule and actin staining by immunofluorescence and LifeAct, dissecting the mating cascade of events, the involvement of Ras, and the specific two-gene encoded dynein in Schizophyllum. The fungis is genetically tractable and, as the only higher basidiomycete model, allows for knock-out mutations.


To address both long-standing questions, we would like to investigate dynein binding proteins (the genome does not contain sequences for some of the proteins involved in nuclear migration in ascomycetes), and to address the potential markers for nuclear identity. While the former question would allow to identify specific evolutionary constraints for nuclear migration and dynein function in higher basidioymcetes, the latter has not been answered for either ascomycetes or basidiomycetes so far.


We expect:


  • a Master's degree (or equivalent allowing you to pursue a PhD degree) in Natural or Life Sciences. Research at ILRS is centred around “Microbial and Biomolecular Interactions”. Candidates about to earn their degree are welcome to apply.

  • experience in microbiology and molecular genetics of fungi

  • high motivation to join one of the research areas of ILRS

  • creativity and interest in shaping your own cross-disciplinary thesis project

  • an integrative and cooperative personality with enthusiasm for actively participating in our lively community

  • very good communication skills in English, the working language at the ILRS.

We offer:


  • a top-level research environment

  • efficient supervision by a team of advisors

  • a comprehensive mentoring programme

  • courses in state-of-the-art technologies and soft skills

  • strong communication and interaction between the involved institutions

  • Jena - City of Science: innovative business activities, successful scientific centres and a vibrant cultural scene around the famous Friedrich Schiller University

The three-year Doctoral Researcher position is available starting from January 2018. The position will be financially supported according to TV-L (salary agreement for public service employees). HKI is an equal opportunity employer.


Further information:

Prof. Dr. Erika Kothe, PI: erika.kothe@uni-jena.de


Dr. Christine Vogler, ILRS Coordinator: ilrs@leibniz-hki.de


The application process is handled exclusively online. Please acquaint yourself with the scientific projects offered on our website. Please note that you can choose up to 3 projects of interest in your application. It is only necessary to fill in the application form for your first preference of projects; the other two projects can be selected in the application form.


Deadline for application: Oct 22, 2017.

Successful applicants will be invited to attend a recruitment meeting in Jena (envisaged date: Dec 14-15, 2017).



This job comes from a partnership with Science Magazine and Euraxess